2j6h
From Proteopedia
(New page: 200px<br /> <applet load="2j6h" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j6h, resolution 2.35Å" /> '''E. COLI GLUCOSAMINE...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2J6H is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with G6Q and ONL as [[http://en.wikipedia.org/wiki/ligands ligands]]. This structure superseeds the now removed PDB entry 2BPJ. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.16 2.6.1.16]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J6H OCA]]. | + | 2J6H is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with G6Q and ONL as [[http://en.wikipedia.org/wiki/ligands ligands]]. This structure superseeds the now removed PDB entry 2BPJ. Active as [[http://en.wikipedia.org/wiki/Glutamine--fructose-6-phosphate_transaminase_(isomerizing) Glutamine--fructose-6-phosphate transaminase (isomerizing)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.16 2.6.1.16]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J6H OCA]]. |
==Reference== | ==Reference== | ||
Glutamine binding opens the ammonia channel and activates glucosamine-6P synthase., Mouilleron S, Badet-Denisot MA, Golinelli-Pimpaneau B, J Biol Chem. 2006 Feb 17;281(7):4404-12. Epub 2005 Dec 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16339762 16339762] | Glutamine binding opens the ammonia channel and activates glucosamine-6P synthase., Mouilleron S, Badet-Denisot MA, Golinelli-Pimpaneau B, J Biol Chem. 2006 Feb 17;281(7):4404-12. Epub 2005 Dec 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16339762 16339762] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| + | [[Category: Glutamine--fructose-6-phosphate transaminase (isomerizing)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Golinelli-Pimpaneau, B.]] | [[Category: Golinelli-Pimpaneau, B.]] | ||
| Line 29: | Line 30: | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:54:42 2007'' |
Revision as of 08:49, 30 October 2007
|
E. COLI GLUCOSAMINE-6-P SYNTHASE IN COMPLEX WITH GLUCOSE-6P AND 5-OXO-L-NORLEUCINE
Overview
Glucosamine-6P synthase catalyzes the synthesis of glucosamine-6P from, fructose-6P and glutamine and uses a channel to transfer ammonia from its, glutaminase to its synthase active site. X-ray structures of, glucosamine-6P synthase have been determined at 2.05 Angstroms resolution, in the presence of fructose-6P and at 2.35 Angstroms resolution in the, presence of fructose-6P and 6-diazo-5-oxo-L-norleucine, a glutamine, affinity analog that covalently modifies the N-terminal catalytic, cysteine, therefore mimicking the gamma-glutamyl-thioester intermediate, formed during hydrolysis of glutamine. The fixation of the glutamine, analog activates the enzyme through several major structural changes: 1), the closure of a loop to shield the glutaminase site accompanied by, significant domain ... [(full description)]
About this Structure
2J6H is a [Single protein] structure of sequence from [Escherichia coli] with G6Q and ONL as [ligands]. This structure superseeds the now removed PDB entry 2BPJ. Active as [Glutamine--fructose-6-phosphate transaminase (isomerizing)], with EC number [2.6.1.16]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Glutamine binding opens the ammonia channel and activates glucosamine-6P synthase., Mouilleron S, Badet-Denisot MA, Golinelli-Pimpaneau B, J Biol Chem. 2006 Feb 17;281(7):4404-12. Epub 2005 Dec 9. PMID:16339762
Page seeded by OCA on Tue Oct 30 10:54:42 2007
Categories: Escherichia coli | Glutamine--fructose-6-phosphate transaminase (isomerizing) | Single protein | Golinelli-Pimpaneau, B. | Mouilleron, S. | G6Q | ONL | 5-oxo-l-norleucine | Amidotransferase | Aminotransferase | Ammonia channeling | Direct protein sequencing | Glucosamine 6-phosphate synthase | Glucose 6-phosphate | Glutamine amidotransferase | N terminal nucleophile | Transferase
