2v2k
From Proteopedia
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| - | [[Image:2v2k. | + | [[Image:2v2k.jpg|left|200px]]<br /><applet load="2v2k" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2v2k" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2v2k, resolution 1.60Å" /> | caption="2v2k, resolution 1.60Å" /> | ||
'''THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS'''<br /> | '''THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2V2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis] with ACT and F3S as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 2V2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis] with ACT and F3S as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:F3s Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2V2K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transport]] | [[Category: transport]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 20:26:32 2007'' |
Revision as of 18:16, 18 December 2007
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THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS
Overview
Mycobacterium smegmatis ferredoxin FdxA, which has an orthologue, ferredoxin in Mycobacterium tuberculosis, FdxC, contains both one [3Fe-4S], and one [4Fe-4S] cluster. M. smegmatis FdxA has been shown to be a, preferred ferredoxin substrate of FprA [F. Fischer, D. Raimondi, A., Aliverti, G. Zanetti, Mycobacterium tuberculosis FprA, a novel bacterial, NADPH-ferredoxin reductase, Eur. J. Biochem. 269 (2002) 3005-3013], an, adrenodoxin reductase-like flavoprotein of M. tuberculosis, suggesting, that M. tuberculosis FdxC could be the physiological partner of the enzyme, in providing reducing power to the cytochromes P450. We report here the, crystal structure of FdxA at 1.6A resolution (R(factor) 16.5%, R(free), 20.2%). Besides providing an insight on protein architecture for this, 106-residue ferredoxin, our crystallographic investigation highlights, lability of the [4Fe-4S] center, which is shown to loose a Fe atom during, crystal growth. Due to their high similarity (87% sequence identity), the, structure here reported can be considered a valuable model for M., tuberculosis FdxC, thus representing a step forward in the study of the, complex mycobacterial redox pathways.
About this Structure
2V2K is a Single protein structure of sequence from Mycobacterium smegmatis with ACT and F3S as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The crystal structure of FdxA, a 7Fe ferredoxin from Mycobacterium smegmatis., Ricagno S, de Rosa M, Aliverti A, Zanetti G, Bolognesi M, Biochem Biophys Res Commun. 2007 Aug 17;360(1):97-102. Epub 2007 Jun 12. PMID:17577575
Page seeded by OCA on Tue Dec 18 20:26:32 2007
Categories: Mycobacterium smegmatis | Single protein | Aliverti, A. | Bolognesi, M. | Ricagno, S. | Rosa, M.De. | Zanetti, G. | ACT | F3S | 3fe-4s | 4fe-4s | 4fe-4s cluster instability | 7fe ferredoxin | Electron transfer | Electron transport | Fe-s cluster | Iron | Iron-sulfur | Metal-binding | Mycobacterium tuberculosis | Transport
