2def
From Proteopedia
(New page: 200px<br /> <applet load="2def" size="450" color="white" frame="true" align="right" spinBox="true" caption="2def" /> '''PEPTIDE DEFORMYLASE CATALYTIC CORE (RESIDUE...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2DEF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with NI as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.31 3.5.1.31]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2DEF OCA]]. | + | 2DEF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with NI as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Formylmethionine_deformylase Formylmethionine deformylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.31 3.5.1.31]]. Structure known Active Site: CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2DEF OCA]]. |
==Reference== | ==Reference== | ||
Solution structure of nickel-peptide deformylase., Dardel F, Ragusa S, Lazennec C, Blanquet S, Meinnel T, J Mol Biol. 1998 Jul 17;280(3):501-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9665852 9665852] | Solution structure of nickel-peptide deformylase., Dardel F, Ragusa S, Lazennec C, Blanquet S, Meinnel T, J Mol Biol. 1998 Jul 17;280(3):501-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9665852 9665852] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| + | [[Category: Formylmethionine deformylase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dardel, F.]] | [[Category: Dardel, F.]] | ||
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[[Category: metalloprotease]] | [[Category: metalloprotease]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:56:52 2007'' |
Revision as of 08:52, 30 October 2007
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PEPTIDE DEFORMYLASE CATALYTIC CORE (RESIDUES 1-147), NMR, 20 STRUCTURES
Overview
In the accompanying paper, we report that zinc is unlikely to be the, co-factor supporting peptide deformylase activity in vivo. In contrast, nickel binding promotes full enzyme activity. The three-dimensional, structure of the resulting nickel-containing peptide deformylase, (catalytic domain, residues 1 to 147) was solved by NMR using a, 13C-15N-doubly labelled protein sample. A set of 2261 restraints could be, collected, with an average of 15.4 per amino acid. The resolution, which, shows a good definition for the position of most side-chains, is greatly, improved compared to that previously reported for the zinc-containing, inactive form. A comparison of the two stuctures indicates however that, both share the same 3D organization. This shows that the nature of the, bound metal is the ... [(full description)]
About this Structure
2DEF is a [Single protein] structure of sequence from [Escherichia coli] with NI as [ligand]. Active as [Formylmethionine deformylase], with EC number [3.5.1.31]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
Reference
Solution structure of nickel-peptide deformylase., Dardel F, Ragusa S, Lazennec C, Blanquet S, Meinnel T, J Mol Biol. 1998 Jul 17;280(3):501-13. PMID:9665852
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