3pcn
From Proteopedia
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| - | [[Image:3pcn.gif|left|200px]]<br /> | + | [[Image:3pcn.gif|left|200px]]<br /><applet load="3pcn" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="3pcn" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="3pcn, resolution 2.4Å" /> | caption="3pcn, resolution 2.4Å" /> | ||
'''STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3,4-DIHYDROXYPHENYLACETATE'''<br /> | '''STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3,4-DIHYDROXYPHENYLACETATE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 3PCN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with FE, BME and DHY as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Protocatechuate_3,4-dioxygenase Protocatechuate 3,4-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.3 1.13.11.3] | + | 3PCN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with FE, BME and DHY as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Protocatechuate_3,4-dioxygenase Protocatechuate 3,4-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.3 1.13.11.3] Known structural/functional Sites: <scene name='pdbsite=ACA:Site Aca Is The Active Site Of Protomer Consisting Of Ch ...'>ACA</scene>, <scene name='pdbsite=ACB:Site Acb Is The Active Site Of Protomer Consisting Of Ch ...'>ACB</scene>, <scene name='pdbsite=ACC:Site Acc Is The Active Site Of Protomer Consisting Of Ch ...'>ACC</scene>, <scene name='pdbsite=ACD:Site Acd Is The Active Site Of Protomer Consisting Of Ch ...'>ACD</scene>, <scene name='pdbsite=ACE:Site Ace Is The Active Site Of Protomer Consisting Of Ch ...'>ACE</scene>, <scene name='pdbsite=ACF:Site Acf Is The Active Site Of Protomer Consisting Of Ch ...'>ACF</scene>, <scene name='pdbsite=VEA:Site Vea Is The Vestigial Site Of Protomer Consisting Of ...'>VEA</scene>, <scene name='pdbsite=VEB:Site Veb Is The Vestigial Site Of Protomer Consisting Of ...'>VEB</scene>, <scene name='pdbsite=VEC:Site Vec Is The Vestigial Site Of Protomer Consisting Of ...'>VEC</scene>, <scene name='pdbsite=VED:Site Ved Is The Vestigial Site Of Protomer Consisting Of ...'>VED</scene>, <scene name='pdbsite=VEE:Site Vee Is The Vestigial Site Of Protomer Consisting Of ...'>VEE</scene> and <scene name='pdbsite=VEF:Site Vef Is The Vestigial Site Of Protomer Consisting Of ...'>VEF</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3PCN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: substrate complex]] | [[Category: substrate complex]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 20:41:35 2007'' |
Revision as of 18:31, 18 December 2007
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STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3,4-DIHYDROXYPHENYLACETATE
Overview
The crystal structure of the anaerobic complex of Pseudomonas putida, protocatechuate 3,4-dioxygenase (3,4-PCD) bound with the alternative, substrate, 3,4-dihydroxyphenylacetate (HPCA), is reported at 2.4 A, resolution and refined to an R factor of 0.17. Formation of the active, site Fe(III).HPCA chelated complex causes the endogenous axial tyrosinate, Tyr447 (147beta), to dissociate from the iron and rotate into an, alternative orientation analogous to that previously observed in the, anaerobic 3,4-PCD.3,4-dihydroxybenzoate complex (3, 4-PCD.PCA) [Orville, A. M., Lipscomb, J. D., & Ohlendorf, D. H. (1997) Biochemistry 36, 10052-10066]. Two orientations of the aromatic ring of HPCA related by an, approximate 180 degrees rotation within the active site are consistent, with the electron density. Resonance Raman (rR) spectroscopic data from, Brevibacteriumfuscum 3,4-PCD.HPCA complex in solution reveals low, frequency rR vibrational bands between 500 and 650 cm-1 as well as a band, at approximately 1320 cm-1 which are diagnostic of a HPCA. Fe(III) chelate, complex. 18O labeling of HPCA at either the C4 or C3 hydroxyl group, unambiguously establishes the vibrational coupling modes associated with, the five-membered chelate ring system. Analysis of these data suggests, that the Fe(III)-HPCAO4 bond is shorter than the Fe(III)-HPCAO3 bond. This, consequently favors the model for the crystal structure in which the C3, phenolic function occupies the Fe3+ ligand site opposite the endogenous, ligand Tyr408(Oeta) (108beta). This is essentially the same binding, orientation as proposed for PCA in the crystal structure of the anaerobic, 3,4-PCD.PCA complex based solely on direct modeling of the 2Fo - Fc, electron density and suggests that this is the conformation required for, catalysis.
About this Structure
3PCN is a Protein complex structure of sequences from Pseudomonas putida with FE, BME and DHY as ligands. Active as Protocatechuate 3,4-dioxygenase, with EC number 1.13.11.3 Known structural/functional Sites: , , , , , , , , , , and . Full crystallographic information is available from OCA.
Reference
Crystal structure and resonance Raman studies of protocatechuate 3,4-dioxygenase complexed with 3,4-dihydroxyphenylacetate., Elgren TE, Orville AM, Kelly KA, Lipscomb JD, Ohlendorf DH, Que L Jr, Biochemistry. 1997 Sep 23;36(38):11504-13. PMID:9298971
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