Ubiquitin

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 14: Line 14:
-
[[2kn5]], [[2klg]], [[2jzz]], [[2pe9]], [[2pea]], [[1g6j]], [[1d3z]] – hUBB – NMR
+
[[2kn5]], [[2klg]], [[2jzz]], [[2pe9]], [[2pea]], [[1g6j]], [[1d3z]] – hUBB – NMR<br />
-
[[2ojr]], [[2nr2]], [[2fcq]], [[1ubi]], [[1ubq]] - hUBB
+
[[2ojr]], [[2nr2]], [[2fcq]], [[1ubi]], [[1ubq]] - hUBB<br />
-
[[2zcb]], [[2gbj]], [[2gbk]], [[2gbm]], [[2gbn]], [[2gbr]], [[2fcm]], [[2fcn]], [[2fcs]], [[1yiw]], [[1yj1]], [[1sif]], [[1ogw]] - hUBB (mutant)
+
[[2zcb]], [[2gbj]], [[2gbk]], [[2gbm]], [[2gbn]], [[2gbr]], [[2fcm]], [[2fcn]], [[2fcs]], [[1yiw]], [[1yj1]], [[1sif]], [[1ogw]] - hUBB (mutant)<br />
-
[[1c3t]], [[1ud7]] - hUBB (mutant) - NMR
+
[[1c3t]], [[1ud7]] - hUBB (mutant) - NMR<br />
-
[[1gjz]] – hUBB N-terminal – NMR
+
[[1gjz]] – hUBB N-terminal – NMR<br />
-
[[1e0q]] - hUBB N-terminal (mutant) – NMR
+
[[1e0q]] - hUBB N-terminal (mutant) – NMR<br />
-
[[2k39]] – UBB – ''Xenopus laevis'' – NMR
+
[[2k39]] – UBB – ''Xenopus laevis'' – NMR<br />
-
[[2jwz]], [[1zw7]] – yUBB (mutant) – yeast
+
[[2jwz]], [[1zw7]] – yUBB (mutant) – yeast<br />
-
[[1cmx]] – yUBB (modified)
+
[[1cmx]] – yUBB (modified)<br />
-
[[2zcc]] – bUBB - bovine
+
[[2zcc]] – bUBB - bovine<br />
Line 29: Line 29:
-
[[3cmm]] – yUBB+E1
+
[[3cmm]] – yUBB+E1 <br />
-
[[3k9p]], [[3a33]] – hUBB+E2
+
[[3k9p]], [[3a33]] – hUBB+E2<br />
-
[[2gmi]] - hUBB+E2+MMS2
+
[[2gmi]] - hUBB+E2+MMS2<br />
-
[[2kjh]], [[1zgu]] – hUBB (mutant)+E2
+
[[2kjh]], [[1zgu]] – hUBB (mutant)+E2<br />
-
[[3jvz]], [[3jw0]] – hUBB+E2+E3
+
[[3jvz]], [[3jw0]] – hUBB+E2+E3<br />
Line 39: Line 39:
-
[[2kx0]] – hUBB+1 – human – NMR
+
[[2kx0]] – hUBB+1 – human – NMR<br />
-
[[3k9o]] – hUBB+1+E2
+
[[3k9o]] – hUBB+1+E2<br />
Line 46: Line 46:
-
[[2xk5]], [[3nob]], [[2xew]], [[3h7p]], [[3h7s]], [[2w9n]], [[2jf5]] – hDiUBB
+
[[2xk5]], [[3nob]], [[2xew]], [[3h7p]], [[3h7s]], [[2w9n]], [[2jf5]] – hDiUBB<br />
-
[[3m3j]], [[1aar]] – bDiUBB
+
[[3m3j]], [[1aar]] – bDiUBB <br />
-
[[2bgf]] – hDiUBB – chemical relaxation
+
[[2bgf]] – hDiUBB – chemical relaxation<br />
Line 54: Line 54:
-
[[3alb]], [[3hm3]], [[2o6v]], [[1f9j]], [[1tbe]] – tetra-hUBB
+
[[3alb]], [[3hm3]], [[2o6v]], [[1f9j]], [[1tbe]] – tetra-hUBB<br />
Line 60: Line 60:
[[3ofi]] – hUBB+insulin protease
[[3ofi]] – hUBB+insulin protease
-
[[3n3k]], [[3ifw]], [[3kvf]], [[3kw5]], [[3irt]], [[3mtn]], [[3ihp]] – hUBB (mutant)+hUBB carboxyl-terminal esterase catalytic domain
+
[[3n3k]], [[3ifw]], [[3kvf]], [[3kw5]], [[3irt]], [[3mtn]], [[3ihp]] – hUBB (mutant)+hUBB carboxyl-terminal esterase catalytic domain<br />
-
[[3i3t]], [[2ibi]], [[2ayo]], [[1nbf]] - hUBB+hUBB carboxyl-terminal hydrolase catalytic domain
+
[[3i3t]], [[2ibi]], [[2ayo]], [[1nbf]] - hUBB+hUBB carboxyl-terminal hydrolase catalytic domain<br />
-
[[2hd5]] - bUBB+hUBB carboxyl-terminal hydrolase catalytic domain
+
[[2hd5]] - bUBB+hUBB carboxyl-terminal hydrolase catalytic domain<br />
-
[[3ldz]] – bUBB+signal transducing adapter molecule
+
[[3ldz]] – bUBB+signal transducing adapter molecule<br />
-
[[2khw]] – hUBB+immunoglobulin G-binding protein G
+
[[2khw]] – hUBB+immunoglobulin G-binding protein G<br />
-
[[2kdi]] – yUBB/UIM fusion
+
[[2kdi]] – yUBB/UIM fusion <br />
-
[[1v80]], [[1v81]] – bUBB/L40 ribosomal protein fusion - NMR
+
[[1v80]], [[1v81]] – bUBB/L40 ribosomal protein fusion - NMR<br />
-
[[1yx5]], [[1yx6]] - hUBB+26S proteasome non-ATPase regulatory subunit – NMR
+
[[1yx5]], [[1yx6]] - hUBB+26S proteasome non-ATPase regulatory subunit – NMR<br />
-
[[2k6d]] – hUBB+SH3 domain-containing kinase-binding protein – NMR
+
[[2k6d]] – hUBB+SH3 domain-containing kinase-binding protein – NMR<br />
-
[[2z59]] – hUBB+mProtein Adhesion-regulating molecule 1
+
[[2z59]] – hUBB+mProtein Adhesion-regulating molecule 1<br />
-
[[2jy6]] – hUBB+hUbiquilin – NMR
+
[[2jy6]] – hUBB+hUbiquilin – NMR<br />
-
3by4, 3c0r – hUBB+yUBB thioesterase
+
[[3by4]], [[3c0r]] – hUBB+yUBB thioesterase<br />
-
2jt4 – yUBB fragment+cytoskeleton assembly control protein
+
[[2jt4]] – yUBB fragment+cytoskeleton assembly control protein<br />
-
2hth – hUBB+vacuolar protein sorting protein
+
[[2hth]] – hUBB+vacuolar protein sorting protein<br />
-
2dx5, 1p3q - bUBB+vacuolar protein sorting protein
+
[[2dx5]], [[1p3q]] - bUBB+vacuolar protein sorting protein<br />
-
1q0w - yUBB+vacuolar protein sorting protein
+
[[1q0w]] - yUBB+vacuolar protein sorting protein<br />
-
2g3q – yUBB+EDE1 UBA
+
[[2g3q]] – yUBB+EDE1 UBA<br />
-
2c7m, 2c7n, 2fid, 2fif – bUBB+RAB guanine nucleotide exchange factor
+
[[2c7m]], [[2c7n]], [[2fid]], [[2fif]] – bUBB+RAB guanine nucleotide exchange factor<br />
-
2d3g – bUBB+HRS-UIM
+
[[2d3g]] – bUBB+HRS-UIM<br />
-
1wrd – bUBB+TOM1 GAT domain
+
[[1wrd]] – bUBB+TOM1 GAT domain<br />
-
1wr6, 1yd8 - bUBB+GGA3 GAT domain
+
[[1wr6]], [[1yd8]] - bUBB+GGA3 GAT domain<br />
-
1wr1 – yUBB+DSK2P UBA
+
[[1wr1]] – yUBB+DSK2P UBA<br />
-
1s1q – hUBB+tumor susceptibility gene 101 protein
+
[[1s1q]] – hUBB+tumor susceptibility gene 101 protein<br />
-
1q5w – hUBB+NPL4 zinc-fingers
+
[[1q5w]] – hUBB+NPL4 zinc-fingers<br />
-
1uzx – bUBB+VPS23 UEV
+
[[1uzx]] – bUBB+VPS23 UEV<br />
-
1otr – yUBB+CUE2
+
[[1otr]] – yUBB+CUE2<br />
Line 91: Line 91:
-
3a9j, 3a9k, 2wwz, 2wx0, 2wx1 – hDiUBB+mitogen-activated protein kinase
+
[[3a9j]], [[3a9k]], [[2wwz]], [[2wx0]], [[2wx1]] – hDiUBB+mitogen-activated protein kinase<br />
-
3jsv, 2zvn, 2zvo – hDiUBB+NF-κ-b essential modulator
+
[[3jsv]], [[2zvn]], [[2zvo]] – hDiUBB+NF-κ-b essential modulator<br />
-
2kde, 2kdf – DiUBB+26S proteasome non-ATPase regulatory subunit – NMR
+
[[2kde]], [[2kdf]] – DiUBB+26S proteasome non-ATPase regulatory subunit – NMR<br />
-
3a1q – mDiUBB+UBB interaction motif-containing protein – mouse
+
[[3a1q]] – mDiUBB+UBB interaction motif-containing protein – mouse<br />
-
3dvg, 3dvn – hDiUBB+IGG1 fab light&heavy chains
+
[[3dvg]], [[3dvn]] – hDiUBB+IGG1 fab light&heavy chains<br />
-
2znv – mDiUBB+hAMSH-like protease
+
[[2znv]] – mDiUBB+hAMSH-like protease<br />
=== Ubiquitin + ions ===
=== Ubiquitin + ions ===
-
3h1u – bUBB+Cd
+
[[3h1u]] – bUBB+Cd<br />
-
3ehv, 3eec, 3efu – hUBB+ions
+
[[3ehv]], [[3eec]], [[3efu]] – hUBB+ions<br />

Revision as of 14:35, 20 September 2010

Image:1d3z.png
NMR Structure of Ubiquitin, 1d3z

Template:STRUCTURE 1d3z

Ubiquitin (UBB) is found in almost all cells. It binds to proteins tagging them for destruction in the proteasome. UBB is activated by the UBB-activating enzymes E1, E2 and E3. UBB+1 is a frameshifted mutant of UBB observed in several diseases. A dimer of UBB (DiUBB) is formed by linkage of K48 to the C-terminus of a second UBB molecule. At least 4 UBB molecules are needed to tag a protein for the proteasome. The images at the left and at the right correspond to one representative Ubiquitin, i.e. the NMR structure of human Ubiquitin (1d3z).

3D Structures of Ubiquitin

Ubiquitin

2kn5, 2klg, 2jzz, 2pe9, 2pea, 1g6j, 1d3z – hUBB – NMR
2ojr, 2nr2, 2fcq, 1ubi, 1ubq - hUBB
2zcb, 2gbj, 2gbk, 2gbm, 2gbn, 2gbr, 2fcm, 2fcn, 2fcs, 1yiw, 1yj1, 1sif, 1ogw - hUBB (mutant)
1c3t, 1ud7 - hUBB (mutant) - NMR
1gjz – hUBB N-terminal – NMR
1e0q - hUBB N-terminal (mutant) – NMR
2k39 – UBB – Xenopus laevis – NMR
2jwz, 1zw7 – yUBB (mutant) – yeast
1cmx – yUBB (modified)
2zcc – bUBB - bovine


Ubiquitin+UBB-activating enzymes

3cmm – yUBB+E1
3k9p, 3a33 – hUBB+E2
2gmi - hUBB+E2+MMS2
2kjh, 1zgu – hUBB (mutant)+E2
3jvz, 3jw0 – hUBB+E2+E3


Ubiquitin+1 mutant

2kx0 – hUBB+1 – human – NMR
3k9o – hUBB+1+E2


Ubiquitin dimer

2xk5, 3nob, 2xew, 3h7p, 3h7s, 2w9n, 2jf5 – hDiUBB
3m3j, 1aar – bDiUBB
2bgf – hDiUBB – chemical relaxation


Ubiquitin tetramer

3alb, 3hm3, 2o6v, 1f9j, 1tbe – tetra-hUBB


Ubiquitin bound to protein

3ofi – hUBB+insulin protease 3n3k, 3ifw, 3kvf, 3kw5, 3irt, 3mtn, 3ihp – hUBB (mutant)+hUBB carboxyl-terminal esterase catalytic domain
3i3t, 2ibi, 2ayo, 1nbf - hUBB+hUBB carboxyl-terminal hydrolase catalytic domain
2hd5 - bUBB+hUBB carboxyl-terminal hydrolase catalytic domain
3ldz – bUBB+signal transducing adapter molecule
2khw – hUBB+immunoglobulin G-binding protein G
2kdi – yUBB/UIM fusion
1v80, 1v81 – bUBB/L40 ribosomal protein fusion - NMR
1yx5, 1yx6 - hUBB+26S proteasome non-ATPase regulatory subunit – NMR
2k6d – hUBB+SH3 domain-containing kinase-binding protein – NMR
2z59 – hUBB+mProtein Adhesion-regulating molecule 1
2jy6 – hUBB+hUbiquilin – NMR
3by4, 3c0r – hUBB+yUBB thioesterase
2jt4 – yUBB fragment+cytoskeleton assembly control protein
2hth – hUBB+vacuolar protein sorting protein
2dx5, 1p3q - bUBB+vacuolar protein sorting protein
1q0w - yUBB+vacuolar protein sorting protein
2g3q – yUBB+EDE1 UBA
2c7m, 2c7n, 2fid, 2fif – bUBB+RAB guanine nucleotide exchange factor
2d3g – bUBB+HRS-UIM
1wrd – bUBB+TOM1 GAT domain
1wr6, 1yd8 - bUBB+GGA3 GAT domain
1wr1 – yUBB+DSK2P UBA
1s1q – hUBB+tumor susceptibility gene 101 protein
1q5w – hUBB+NPL4 zinc-fingers
1uzx – bUBB+VPS23 UEV
1otr – yUBB+CUE2


Ubiquitin dimer bound to protein

3a9j, 3a9k, 2wwz, 2wx0, 2wx1 – hDiUBB+mitogen-activated protein kinase
3jsv, 2zvn, 2zvo – hDiUBB+NF-κ-b essential modulator
2kde, 2kdf – DiUBB+26S proteasome non-ATPase regulatory subunit – NMR
3a1q – mDiUBB+UBB interaction motif-containing protein – mouse
3dvg, 3dvn – hDiUBB+IGG1 fab light&heavy chains
2znv – mDiUBB+hAMSH-like protease


Ubiquitin + ions

3h1u – bUBB+Cd
3ehv, 3eec, 3efu – hUBB+ions

Personal tools