2j4r
From Proteopedia
(New page: 200px<br /><applet load="2j4r" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j4r, resolution 2.71Å" /> '''STRUCTURAL STUDY OF ...) |
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| - | [[Image:2j4r.gif|left|200px]]<br /><applet load="2j4r" size=" | + | [[Image:2j4r.gif|left|200px]]<br /><applet load="2j4r" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2j4r, resolution 2.71Å" /> | caption="2j4r, resolution 2.71Å" /> | ||
'''STRUCTURAL STUDY OF THE AQUIFEX AEOLICUS PPX-GPPA ENZYME'''<br /> | '''STRUCTURAL STUDY OF THE AQUIFEX AEOLICUS PPX-GPPA ENZYME'''<br /> | ||
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| + | ==Overview== | ||
| + | The crystal structure of the prototype exopolyphosphatase/guanosine, pentaphosphate phosphohydrolase protein family member from Aquifex, aeolicus in complex with the intracellular second messenger guanosine, tetraphosphate was determined at 2.7-A resolution. The hydrolytic base is, identified as E119. The dual specificity established for the Escherichia, coli homolog is shown to be compatible with a common active site for, guanosine pentaphosphate and polyphosphate hydrolysis. Distinct and, different degrees of closure between the two domains of the enzyme are, associated with substrate binding. The arginines R22 and R267, residing in, different domains, are crucial for guanosine pentaphosphate specificity as, they interact with the unique 3'-ribose phosphorylation. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2J4R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with G4P as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:G4p Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2J4R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with <scene name='pdbligand=G4P:'>G4P</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:G4p Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J4R OCA]. |
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| + | ==Reference== | ||
| + | Structure of the PPX/GPPA phosphatase from Aquifex aeolicus in complex with the alarmone ppGpp., Kristensen O, Ross B, Gajhede M, J Mol Biol. 2008 Feb 1;375(5):1469-76. Epub 2007 Dec 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18155044 18155044] | ||
[[Category: Aquifex aeolicus]] | [[Category: Aquifex aeolicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: stringent response]] | [[Category: stringent response]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 10:38:38 2008'' |
Revision as of 08:38, 23 January 2008
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STRUCTURAL STUDY OF THE AQUIFEX AEOLICUS PPX-GPPA ENZYME
Overview
The crystal structure of the prototype exopolyphosphatase/guanosine, pentaphosphate phosphohydrolase protein family member from Aquifex, aeolicus in complex with the intracellular second messenger guanosine, tetraphosphate was determined at 2.7-A resolution. The hydrolytic base is, identified as E119. The dual specificity established for the Escherichia, coli homolog is shown to be compatible with a common active site for, guanosine pentaphosphate and polyphosphate hydrolysis. Distinct and, different degrees of closure between the two domains of the enzyme are, associated with substrate binding. The arginines R22 and R267, residing in, different domains, are crucial for guanosine pentaphosphate specificity as, they interact with the unique 3'-ribose phosphorylation.
About this Structure
2J4R is a Single protein structure of sequence from Aquifex aeolicus with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of the PPX/GPPA phosphatase from Aquifex aeolicus in complex with the alarmone ppGpp., Kristensen O, Ross B, Gajhede M, J Mol Biol. 2008 Feb 1;375(5):1469-76. Epub 2007 Dec 4. PMID:18155044
Page seeded by OCA on Wed Jan 23 10:38:38 2008
