2jmh
From Proteopedia
(New page: 200px<br /> <applet load="2jmh" size="450" color="white" frame="true" align="right" spinBox="true" caption="2jmh" /> '''NMR solution structure of Blo t 5, a major ...) |
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'''NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis'''<br /> | '''NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis'''<br /> | ||
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| + | ==Overview== | ||
| + | Blo t 5 is the major allergen from Blomia tropicalis mites and shows, strong IgE reactivity with up to 90% of asthmatic and rhinitis patients', sera. The NMR solution structure of Blo t 5 comprises three long alpha, helices, forming a coiled-coil, triple-helical bundle with a left-handed, twist. TROSY-NMR experiments were used to study Blo t 5 interaction with, the Fab' of a specific monoclonal antibody, mAb 4A7. The mAb epitope, comprises two closely spaced surfaces, I and II, connected by a sharp turn, and bearing critical residues Asn46 and Lys47 on one surface, and Lys54, and Arg57 on the other. This discontinuous epitope overlaps with the human, IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to, undergo conformational exchange in the millisecond to microsecond range., The results presented are critical for the design of a hypoallergenic Blo, t 5 for efficacious immunotherapy of allergic diseases. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2JMH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Blomia_tropicalis Blomia tropicalis]. Full crystallographic information is available from [http:// | + | 2JMH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Blomia_tropicalis Blomia tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMH OCA]. |
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| + | ==Reference== | ||
| + | Roles of Structure and Structural Dynamics in the Antibody Recognition of the Allergen Proteins: An NMR Study on Blomia tropicalis Major Allergen., Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH, Structure. 2008 Jan;16(1):125-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18184590 18184590] | ||
[[Category: Blomia tropicalis]] | [[Category: Blomia tropicalis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: group 5]] | [[Category: group 5]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 10:39:13 2008'' |
Revision as of 08:39, 23 January 2008
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NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis
Overview
Blo t 5 is the major allergen from Blomia tropicalis mites and shows, strong IgE reactivity with up to 90% of asthmatic and rhinitis patients', sera. The NMR solution structure of Blo t 5 comprises three long alpha, helices, forming a coiled-coil, triple-helical bundle with a left-handed, twist. TROSY-NMR experiments were used to study Blo t 5 interaction with, the Fab' of a specific monoclonal antibody, mAb 4A7. The mAb epitope, comprises two closely spaced surfaces, I and II, connected by a sharp turn, and bearing critical residues Asn46 and Lys47 on one surface, and Lys54, and Arg57 on the other. This discontinuous epitope overlaps with the human, IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to, undergo conformational exchange in the millisecond to microsecond range., The results presented are critical for the design of a hypoallergenic Blo, t 5 for efficacious immunotherapy of allergic diseases.
About this Structure
2JMH is a Single protein structure of sequence from Blomia tropicalis. Full crystallographic information is available from OCA.
Reference
Roles of Structure and Structural Dynamics in the Antibody Recognition of the Allergen Proteins: An NMR Study on Blomia tropicalis Major Allergen., Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH, Structure. 2008 Jan;16(1):125-36. PMID:18184590
Page seeded by OCA on Wed Jan 23 10:39:13 2008
Categories: Blomia tropicalis | Single protein | Chang, C. | Chua, K. | Huang, T. | Kuo, I. | Naik, M.T. | Allergen | Blo t 5 | Dust mites | Group 5
