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2q5d

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(New page: 200px<br /><applet load="2q5d" size="350" color="white" frame="true" align="right" spinBox="true" caption="2q5d, resolution 3.2&Aring;" /> '''Crystal Structure of ...)
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Revision as of 08:52, 23 January 2008

Image:2q5d.jpg

2q5d, resolution 3.2Å

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Crystal Structure of Human Importin Beta bound to the Snurportin1 IBB-domain second crystal form

Overview

The nuclear import of uridine-rich ribonucleoproteins (U snRNPs) is, mediated by the transport adaptor snurportin 1 (SNP1). Similar to importin, alpha, SNP1 uses an N-terminal importin beta binding (sIBB) domain to, recruit the receptor importin beta and gain access to the nucleus. In this, paper, we demonstrate the sIBB-domain has a bipartite nature, which, contains two distinct binding determinants for importin beta. The first, determinant spans residues 25-65, and includes the previously identified, alphaIBB-region of homology. The second binding determinant encompasses, residues 1-24 and resembles region 1011-1035 of the nucleoporin 153, (Nup153). The two binding determinants synergize within the sIBB-domain to, confer a low nanomolar binding affinity for importin beta, (Kd ~2nM) in an, interaction that, in vitro, is displaced by RanGppNHp. We propose that in, vivo the synergy of Nup153 and nuclear RanGTP promotes translocation of U, snRNPs into the nucleus.

About this Structure

2Q5D is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Molecular basis for the recognition of snurportin 1 by importin beta., Mitrousis G, Olia AS, Walker-Kopp N, Cingolani G, J Biol Chem. 2008 Jan 9;. PMID:18187419

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