1iov
From Proteopedia
(New page: 200px<br /> <applet load="1iov" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iov, resolution 2.2Å" /> '''COMPLEX OF D-ALA:D-A...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1IOV is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MG, ADP and POB as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.4 6.3.2.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IOV OCA]]. | + | 1IOV is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MG, ADP and POB as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/D-alanine--D-alanine_ligase D-alanine--D-alanine ligase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.4 6.3.2.4]]. Structure known Active Sites: ADP and LIG. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IOV OCA]]. |
==Reference== | ==Reference== | ||
D-alanine:D-alanine ligase: phosphonate and phosphinate intermediates with wild type and the Y216F mutant., Fan C, Park IS, Walsh CT, Knox JR, Biochemistry. 1997 Mar 4;36(9):2531-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9054558 9054558] | D-alanine:D-alanine ligase: phosphonate and phosphinate intermediates with wild type and the Y216F mutant., Fan C, Park IS, Walsh CT, Knox JR, Biochemistry. 1997 Mar 4;36(9):2531-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9054558 9054558] | ||
| + | [[Category: D-alanine--D-alanine ligase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: vancomycin]] | [[Category: vancomycin]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:02:08 2007'' |
Revision as of 08:57, 30 October 2007
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COMPLEX OF D-ALA:D-ALA LIGASE WITH ADP AND A PHOSPHORYL PHOSPHONATE
Overview
The crystallographic structure of the D-alanine:D-alanine ligase of the, ddlB gene of Escherichia coli complexed with a, D-Ala-D-alpha-hydroxybutyrate phosphonate and the structure of the Y216F, mutant ligase complexed with a D-Ala-D-Ala phosphinate have been, determined to 2.2 and 1.9 A resolution, respectively, and refined to R, factors of 0.156 and 0.158. In each complex the inhibitor has reacted with, ATP to produce ADP and a tight-binding phosphorylated transition state, intermediate. Comparison of these two structures with the known crystal, structure of the phosphinate intermediate of the wild-type ligase shows no, major conformational changes, but B factors indicate differences in, mobility of loops covering the binding site. The weaker inhibition of the, Y216F mutant by both ... [(full description)]
About this Structure
1IOV is a [Single protein] structure of sequence from [Escherichia coli] with MG, ADP and POB as [ligands]. Active as [D-alanine--D-alanine ligase], with EC number [6.3.2.4]. Structure known Active Sites: ADP and LIG. Full crystallographic information is available from [OCA].
Reference
D-alanine:D-alanine ligase: phosphonate and phosphinate intermediates with wild type and the Y216F mutant., Fan C, Park IS, Walsh CT, Knox JR, Biochemistry. 1997 Mar 4;36(9):2531-8. PMID:9054558
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