2q8y

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(New page: 200px<br /><applet load="2q8y" size="350" color="white" frame="true" align="right" spinBox="true" caption="2q8y, resolution 2.00&Aring;" /> '''Structural insight i...)
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Revision as of 09:02, 23 January 2008


2q8y, resolution 2.00Å

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Structural insight into the enzymatic mechanism of the phophothreonine lyase

Overview

The OspF family of phosphothreonine lyase, including SpvC from Salmonella, irreversibly inactivates the dual-phosphorylated host MAPKs (pT-X-pY), through beta elimination. We determined crystal structures of SpvC and its, complex with a phosphopeptide substrate. SpvC adopts a unique fold of, alpha/beta type. The disordered N terminus harbors a canonical D motif for, MAPK substrate docking. The enzyme-substrate complex structure indicates, that recognition of the phosphotyrosine followed by insertion of the, threonine phosphate into an arginine pocket places the phosphothreonine, into the enzyme active site. This requires the conformational flexibility, of pT-X-pY, which suggests that p38 (pT-G-pY) is likely the preferred, physiological substrate. Structure-based biochemical and enzymatic, analysis allows us to propose a general acid/base mechanism for beta, elimination reaction catalyzed by the phosphothreonine lyase. The, mechanism described here provides a structural understanding of MAPK, inactivation by a family of pathogenic effectors conserved in plant and, animal systems and may also open a new route for biological catalysis.

About this Structure

2Q8Y is a Protein complex structure of sequences from Salmonella enteritidis. Active as Lyase, with EC number 4.2. Full crystallographic information is available from OCA.

Reference

Structural insights into the enzymatic mechanism of the pathogenic MAPK phosphothreonine lyase., Zhu Y, Li H, Long C, Hu L, Xu H, Liu L, Chen S, Wang DC, Shao F, Mol Cell. 2007 Dec 14;28(5):899-913. Epub 2007 Nov 29. PMID:18060821

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