2vce
From Proteopedia
(New page: 200px<br /><applet load="2vce" size="450" color="white" frame="true" align="right" spinBox="true" caption="2vce, resolution 1.90Å" /> '''CHARACTERIZATION AND...) |
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| - | [[Image:2vce.gif|left|200px]]<br /><applet load="2vce" size=" | + | [[Image:2vce.gif|left|200px]]<br /><applet load="2vce" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2vce, resolution 1.90Å" /> | caption="2vce, resolution 1.90Å" /> | ||
'''CHARACTERIZATION AND ENGINEERING OF THE BIFUNCTIONAL N- AND O-GLUCOSYLTRANSFERASE INVOLVED IN XENOBIOTIC METABOLISM IN PLANTS'''<br /> | '''CHARACTERIZATION AND ENGINEERING OF THE BIFUNCTIONAL N- AND O-GLUCOSYLTRANSFERASE INVOLVED IN XENOBIOTIC METABOLISM IN PLANTS'''<br /> | ||
| + | |||
| + | ==Overview== | ||
| + | The glucosylation of pollutant and pesticide metabolites in plants, controls their bioactivity and the formation of subsequent chemical, residues. The model plant Arabidopsis thaliana contains >100, glycosyltransferases (GTs) dedicated to small-molecule conjugation and, whereas 44 of these enzymes catalyze the O-glucosylation of chlorinated, phenols, only one, UGT72B1, shows appreciable N-glucosylating activity, toward chloroanilines. UGT72B1 is a bifunctional O-glucosyltransferase, (OGT) and N-glucosyltransferase (NGT). To investigate this unique dual, activity, the structure of the protein was solved, at resolutions up to, 1.45 A, in various forms including the Michaelis complex with intact donor, analog and trichlorophenol acceptor. The catalytic mechanism and basis for, O/N specificity was probed by mutagenesis and domain shuffling with an, orthologous enzyme from Brassica napus (BnUGT), which possesses only OGT, activity. Mutation of BnUGT at just two positions (D312N and F315Y), installed high levels of NGT activity. Molecular modeling revealed the, connectivity of these residues to H19 on UGT72B1, with its mutagenesis, exclusively defining NGT activity in the Arabidopsis enzyme. These results, shed light on the conjugation of nonnatural substrates by plant GTs, highlighting the catalytic plasticity of this enzyme class and the ability, to engineer unusual and desirable transfer to nitrogen-based acceptors. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2VCE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with U2F, TC7 and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Hydroquinone_glucosyltransferase Hydroquinone glucosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.218 2.4.1.218] Known structural/functional | + | 2VCE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with <scene name='pdbligand=U2F:'>U2F</scene>, <scene name='pdbligand=TC7:'>TC7</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Hydroquinone_glucosyltransferase Hydroquinone glucosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.218 2.4.1.218] Known structural/functional Sites: <scene name='pdbsite=AC1:U2f Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:Tc7 Binding Site For Chain A'>AC2</scene> and <scene name='pdbsite=AC3:Edo Binding Site For Chain A'>AC3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VCE OCA]. |
| + | |||
| + | ==Reference== | ||
| + | Characterization and engineering of the bifunctional N- and O-glucosyltransferase involved in xenobiotic metabolism in plants., Brazier-Hicks M, Offen WA, Gershater MC, Revett TJ, Lim EK, Bowles DJ, Davies GJ, Edwards R, Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20238-43. Epub 2007 Dec 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18077347 18077347] | ||
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
[[Category: Hydroquinone glucosyltransferase]] | [[Category: Hydroquinone glucosyltransferase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bowles, D.]] | + | [[Category: Bowles, D.J.]] |
[[Category: Brazier-Hicks, M.]] | [[Category: Brazier-Hicks, M.]] | ||
[[Category: Davies, G.J.]] | [[Category: Davies, G.J.]] | ||
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[[Category: Gershater, M.C.]] | [[Category: Gershater, M.C.]] | ||
[[Category: Lim, E.K.]] | [[Category: Lim, E.K.]] | ||
| - | [[Category: Offen, W.]] | + | [[Category: Offen, W.A.]] |
| - | [[Category: Revett, T.]] | + | [[Category: Revett, T.J.]] |
[[Category: EDO]] | [[Category: EDO]] | ||
[[Category: TC7]] | [[Category: TC7]] | ||
| Line 29: | Line 35: | ||
[[Category: udp-glucose- dependent]] | [[Category: udp-glucose- dependent]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:14:00 2008'' |
Revision as of 09:14, 23 January 2008
|
CHARACTERIZATION AND ENGINEERING OF THE BIFUNCTIONAL N- AND O-GLUCOSYLTRANSFERASE INVOLVED IN XENOBIOTIC METABOLISM IN PLANTS
Overview
The glucosylation of pollutant and pesticide metabolites in plants, controls their bioactivity and the formation of subsequent chemical, residues. The model plant Arabidopsis thaliana contains >100, glycosyltransferases (GTs) dedicated to small-molecule conjugation and, whereas 44 of these enzymes catalyze the O-glucosylation of chlorinated, phenols, only one, UGT72B1, shows appreciable N-glucosylating activity, toward chloroanilines. UGT72B1 is a bifunctional O-glucosyltransferase, (OGT) and N-glucosyltransferase (NGT). To investigate this unique dual, activity, the structure of the protein was solved, at resolutions up to, 1.45 A, in various forms including the Michaelis complex with intact donor, analog and trichlorophenol acceptor. The catalytic mechanism and basis for, O/N specificity was probed by mutagenesis and domain shuffling with an, orthologous enzyme from Brassica napus (BnUGT), which possesses only OGT, activity. Mutation of BnUGT at just two positions (D312N and F315Y), installed high levels of NGT activity. Molecular modeling revealed the, connectivity of these residues to H19 on UGT72B1, with its mutagenesis, exclusively defining NGT activity in the Arabidopsis enzyme. These results, shed light on the conjugation of nonnatural substrates by plant GTs, highlighting the catalytic plasticity of this enzyme class and the ability, to engineer unusual and desirable transfer to nitrogen-based acceptors.
About this Structure
2VCE is a Single protein structure of sequence from Arabidopsis thaliana with , and as ligands. Active as Hydroquinone glucosyltransferase, with EC number 2.4.1.218 Known structural/functional Sites: , and . Full crystallographic information is available from OCA.
Reference
Characterization and engineering of the bifunctional N- and O-glucosyltransferase involved in xenobiotic metabolism in plants., Brazier-Hicks M, Offen WA, Gershater MC, Revett TJ, Lim EK, Bowles DJ, Davies GJ, Edwards R, Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20238-43. Epub 2007 Dec 12. PMID:18077347
Page seeded by OCA on Wed Jan 23 11:14:00 2008
Categories: Arabidopsis thaliana | Hydroquinone glucosyltransferase | Single protein | Bowles, D.J. | Brazier-Hicks, M. | Davies, G.J. | Edwards, R. | Gershater, M.C. | Lim, E.K. | Offen, W.A. | Revett, T.J. | EDO | TC7 | U2F | Glycosyltransferase | N-glucosyltransferase | N-glycosylation | O- glucosyltransferase | O-glycosylation | Plant glycosylation | S-glucosyltransferase | Transferase | Udp-glucose- dependent
