2bw7

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(New page: 200px<br /> <applet load="2bw7" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bw7, resolution 2.3&Aring;" /> '''A NOVEL MECHANISM FO...)
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==About this Structure==
==About this Structure==
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2BW7 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Spirulina_platensis Spirulina platensis]] with MG, CA, APC and ECS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BW7 OCA]].
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2BW7 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Arthrospira_platensis Arthrospira platensis]] with MG, CA, APC and ECS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BW7 OCA]].
==Reference==
==Reference==
A novel mechanism for adenylyl cyclase inhibition from the crystal structure of its complex with catechol estrogen., Steegborn C, Litvin TN, Hess KC, Capper AB, Taussig R, Buck J, Levin LR, Wu H, J Biol Chem. 2005 Sep 9;280(36):31754-9. Epub 2005 Jul 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16002394 16002394]
A novel mechanism for adenylyl cyclase inhibition from the crystal structure of its complex with catechol estrogen., Steegborn C, Litvin TN, Hess KC, Capper AB, Taussig R, Buck J, Levin LR, Wu H, J Biol Chem. 2005 Sep 9;280(36):31754-9. Epub 2005 Jul 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16002394 16002394]
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[[Category: Adenylate cyclase]]
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[[Category: Arthrospira platensis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Spirulina platensis]]
 
[[Category: Buck, J.]]
[[Category: Buck, J.]]
[[Category: Capper, A.B.]]
[[Category: Capper, A.B.]]
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[[Category: oxidoreductase]]
[[Category: oxidoreductase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 17:17:53 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:04:59 2007''

Revision as of 09:00, 30 October 2007


2bw7, resolution 2.3Å

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A NOVEL MECHANISM FOR ADENYLYL CYCLASE INHIBITION FROM THE CRYSTAL STRUCTURE OF ITS COMPLEX WITH CATECHOL ESTROGEN

Overview

Catechol estrogens are steroid metabolites that elicit physiological, responses through binding to a variety of cellular targets. We show here, that catechol estrogens directly inhibit soluble adenylyl cyclases and the, abundant trans-membrane adenylyl cyclases. Catechol estrogen inhibition is, non-competitive with respect to the substrate ATP, and we solved the, crystal structure of a catechol estrogen bound to a soluble adenylyl, cyclase from Spirulina platensis in complex with a substrate analog. The, catechol estrogen is bound to a newly identified, conserved hydrophobic, patch near the active center but distinct from the ATP-binding cleft., Inhibitor binding leads to a chelating interaction between the catechol, estrogen hydroxyl groups and the catalytic magnesium ion, distorting ... [(full description)]

About this Structure

2BW7 is a [Single protein] structure of sequence from [Arthrospira platensis] with MG, CA, APC and ECS as [ligands]. Active as [Adenylate cyclase], with EC number [4.6.1.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

A novel mechanism for adenylyl cyclase inhibition from the crystal structure of its complex with catechol estrogen., Steegborn C, Litvin TN, Hess KC, Capper AB, Taussig R, Buck J, Levin LR, Wu H, J Biol Chem. 2005 Sep 9;280(36):31754-9. Epub 2005 Jul 7. PMID:16002394

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