2vba

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spinqualitylabelsall models 2vba, resolution 1.36Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

BETA-KETOACYL-ACP SYNTHASE I (KAS) FROM E. COLI WITH BOUND AMINO-THIAZOLE INHIBITOR

Overview

Fatty-acid synthesis in bacteria is of great interest as a target for the, discovery of antibacterial compounds. The addition of a new acetyl moiety, to the growing fatty-acid chain, an essential step in this process, is, catalyzed by beta-ketoacyl-ACP synthase (KAS). It is inhibited by natural, antibiotics such as cerulenin and thiolactomycin; however, these lack the, requirements for optimal drug development. Structure-based biophysical, screening revealed a novel synthetic small molecule, 2-phenylamino-4-methyl-5-acetylthiazole, that binds to Escherichia coli, KAS I with a binding constant of 25 microM as determined by fluorescence, titration. A 1.35 A crystal structure of its complex with its target, reveals noncovalent interactions with the active-site Cys163 and, hydrophobic residues of the fatty-acid binding pocket. The active site is, accessible through an open conformation of the Phe392 side chain and no, conformational changes are induced at the active site upon ligand binding., This represents a novel binding mode that differs from thiolactomycin or, cerulenin interaction. The structural information on the protein-ligand, interaction offers strategies for further optimization of this, low-molecular-weight compound.

About this Structure

2VBA is a Single protein structure of sequence from Escherichia coli with as ligand. Active as Beta-ketoacyl-acyl-carrier-protein synthase I, with EC number 2.3.1.41 Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Structure-assisted discovery of an aminothiazole derivative as a lead molecule for inhibition of bacterial fatty-acid synthesis., Pappenberger G, Schulz-Gasch T, Kusznir E, Muller F, Hennig M, Acta Crystallogr D Biol Crystallogr. 2007 Dec;63(Pt 12):1208-16. Epub 2007, Nov 16. PMID:18084068

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