2gaa
From Proteopedia
(New page: 200px<br /><applet load="2gaa" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gaa, resolution 1.95Å" /> '''Crystal structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2gaa.gif|left|200px]]<br /><applet load="2gaa" size=" | + | [[Image:2gaa.gif|left|200px]]<br /><applet load="2gaa" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2gaa, resolution 1.95Å" /> | caption="2gaa, resolution 1.95Å" /> | ||
'''Crystal structure of YFH7 from Saccharomyces cerevisiae: a putative P-loop containing kinase with a circular permutation.'''<br /> | '''Crystal structure of YFH7 from Saccharomyces cerevisiae: a putative P-loop containing kinase with a circular permutation.'''<br /> | ||
| + | |||
| + | ==Overview== | ||
| + | Genome sequencing projects have revealed that P-loop proteins are highly, represented in all organisms and that many of them have no attributed, function. They are characterized by a conserved nucleotide-binding domain, and carry different activities implicated in many cellular processes., Saccharomyces cerevisiae YFH7 is one of these P-loop proteins of unknown, function. In this work we tried to integrate bioinformatics, structure, and enzymology to discover the function of YFH7. Sequence analysis, revealed that yeast YFH7 is a yeast-specific protein showing weak, similarity with the phosphoribulokinase/uridine kinase/bacterial, pantothenate kinase (PRK/URK/PANK) subfamily of P-loop containing kinases., A large insertion of about 100 residues distinguishes YFH7 from other, members of the family. The 1.95 A resolution crystal structure of YFH7, solved using the SAD method confirmed that YFH7 has a fold similar to the, PRK/URK/PANK family, with the characteristic core, lid, and NMP(bind), domains. An additional alpha/beta domain of novel topology corresponds to, the large sequence insertion. Structural and ligand binding analysis, combined with enzymatic assays suggest that YFH7 is an ATP-dependent small, molecule kinase with new substrate specificity. Proteins 2007. (c) 2007, Wiley-Liss, Inc. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2GAA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2GAA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GAA OCA]. |
| + | |||
| + | ==Reference== | ||
| + | Crystal structure and functional analysis identify the P-loop containing protein YFH7 of Saccharomyces cerevisiae as an ATP-dependent kinase., Gueguen-Chaignon V, Chaptal V, Lariviere L, Costa N, Lopes P, Morera S, Nessler S, Proteins. 2007 Nov 14;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18004758 18004758] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 10: | Line 16: | ||
[[Category: Morera, S.]] | [[Category: Morera, S.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
| + | [[Category: unknown function]] | ||
[[Category: yfh7]] | [[Category: yfh7]] | ||
[[Category: yfr007w]] | [[Category: yfr007w]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:30:48 2008'' |
Revision as of 09:30, 23 January 2008
|
Crystal structure of YFH7 from Saccharomyces cerevisiae: a putative P-loop containing kinase with a circular permutation.
Overview
Genome sequencing projects have revealed that P-loop proteins are highly, represented in all organisms and that many of them have no attributed, function. They are characterized by a conserved nucleotide-binding domain, and carry different activities implicated in many cellular processes., Saccharomyces cerevisiae YFH7 is one of these P-loop proteins of unknown, function. In this work we tried to integrate bioinformatics, structure, and enzymology to discover the function of YFH7. Sequence analysis, revealed that yeast YFH7 is a yeast-specific protein showing weak, similarity with the phosphoribulokinase/uridine kinase/bacterial, pantothenate kinase (PRK/URK/PANK) subfamily of P-loop containing kinases., A large insertion of about 100 residues distinguishes YFH7 from other, members of the family. The 1.95 A resolution crystal structure of YFH7, solved using the SAD method confirmed that YFH7 has a fold similar to the, PRK/URK/PANK family, with the characteristic core, lid, and NMP(bind), domains. An additional alpha/beta domain of novel topology corresponds to, the large sequence insertion. Structural and ligand binding analysis, combined with enzymatic assays suggest that YFH7 is an ATP-dependent small, molecule kinase with new substrate specificity. Proteins 2007. (c) 2007, Wiley-Liss, Inc.
About this Structure
2GAA is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure and functional analysis identify the P-loop containing protein YFH7 of Saccharomyces cerevisiae as an ATP-dependent kinase., Gueguen-Chaignon V, Chaptal V, Lariviere L, Costa N, Lopes P, Morera S, Nessler S, Proteins. 2007 Nov 14;. PMID:18004758
Page seeded by OCA on Wed Jan 23 11:30:48 2008
