2jpn
From Proteopedia
(New page: 200px<br /><applet load="2jpn" size="450" color="white" frame="true" align="right" spinBox="true" caption="2jpn" /> '''Solution Structure of T4 Bacteriophage Helic...) |
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| - | [[Image:2jpn.jpg|left|200px]]<br /><applet load="2jpn" size=" | + | [[Image:2jpn.jpg|left|200px]]<br /><applet load="2jpn" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2jpn" /> | caption="2jpn" /> | ||
'''Solution Structure of T4 Bacteriophage Helicase Uvsw.1'''<br /> | '''Solution Structure of T4 Bacteriophage Helicase Uvsw.1'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2JPN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 2JPN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JPN OCA]. |
==Reference== | ==Reference== | ||
Crystallographic and NMR analyses of UvsW and UvsW.1 from bacteriophage T4., Kerr ID, Sivakolundu S, Li Z, Buchsbaum JC, Knox LA, Kriwacki RW, White SW, J Biol Chem. 2007 Sep 17;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17878153 17878153] | Crystallographic and NMR analyses of UvsW and UvsW.1 from bacteriophage T4., Kerr ID, Sivakolundu S, Li Z, Buchsbaum JC, Knox LA, Kriwacki RW, White SW, J Biol Chem. 2007 Sep 17;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17878153 17878153] | ||
| - | [[Category: | + | [[Category: Bacteriophage t4]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Kriwacki, R.W.]] | [[Category: Kriwacki, R.W.]] | ||
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[[Category: White, S.W.]] | [[Category: White, S.W.]] | ||
[[Category: bacteriophage helicase]] | [[Category: bacteriophage helicase]] | ||
| + | [[Category: hydrolase]] | ||
[[Category: uvsw]] | [[Category: uvsw]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:31:15 2008'' |
Revision as of 09:31, 23 January 2008
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Solution Structure of T4 Bacteriophage Helicase Uvsw.1
Overview
The uvsWXY system is implicated in the replication and repair of the, bacteriophage T4 genome. Whilst the roles of the recombinase (UvsX) and, the recombination mediator protein (UvsY) are known, the precise role of, UvsW is unclear. Sequence analysis identifies UvsW as a member of the, monomeric SF2 helicase superfamily that translocates nucleic acid, substrates via the action of two RecA-like motor domains. Functional, homologies to E. coli RecG and biochemical analyses have shown that UvsW, interacts with branched nucleic acid substrates, suggesting roles in, recombination and the rescue of stalled replication forks. A sequencing, error at the 3' end of the uvsW gene has revealed a second, short open, reading frame that encodes a protein of unknown function called UvsW.1. We, have determined the crystal structure of UvsW to 2.7A and the NMR solution, structure of UvsW.1. UvsW has a four-domain architecture with structural, homology to the eukaryotic SF2 helicase, Rad54. A model of the UvsW-ssDNA, complex identifies structural elements and conserved residues that may, interact with nucleic acid substrates. The NMR solution structure of, UvsW.1 reveals a dynamic four-helix bundle with homology to the, structure-specific nucleic acid binding module of RecQ helicases.
About this Structure
2JPN is a Single protein structure of sequence from Bacteriophage t4. Full crystallographic information is available from OCA.
Reference
Crystallographic and NMR analyses of UvsW and UvsW.1 from bacteriophage T4., Kerr ID, Sivakolundu S, Li Z, Buchsbaum JC, Knox LA, Kriwacki RW, White SW, J Biol Chem. 2007 Sep 17;. PMID:17878153
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