2pk9
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(New page: 200px<br /><applet load="2pk9" size="350" color="white" frame="true" align="right" spinBox="true" caption="2pk9, resolution 2.906Å" /> '''Structure of the Ph...)
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Revision as of 09:43, 23 January 2008
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Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway
Overview
The ability to sense and respond appropriately to environmental changes is, a primary requirement of all living organisms. In response to phosphate, limitation, Saccharomyces cerevisiae induces transcription of a set of, genes involved in the regulation of phosphate acquisition from the ambient, environment. A signal transduction pathway (the PHO pathway) mediates this, response, with Pho85-Pho80 playing a vital role. Here we report the X-ray, structure of Pho85-Pho80, a prototypic structure of a CDK-cyclin complex, functioning in transcriptional regulation in response to environmental, changes. The structure revealed a specific salt link between a Pho85, arginine and a Pho80 aspartate that makes phosphorylation of the Pho85, activation loop dispensable and that maintains a Pho80 loop conformation, for possible substrate recognition. It further showed two sites on the, Pho80 cyclin for high-affinity binding of the transcription factor, substrate (Pho4) and the CDK inhibitor (Pho81) that are markedly distant, to each other and the active site.
About this Structure
2PK9 is a Protein complex structure of sequences from Saccharomyces cerevisiae with as ligand. Active as Cyclin-dependent kinase, with EC number 2.7.11.22 Full crystallographic information is available from OCA.
Reference
Structure of the Pho85-Pho80 CDK-Cyclin Complex of the Phosphate-Responsive Signal Transduction Pathway., Huang K, Ferrin-O'Connell I, Zhang W, Leonard GA, O'Shea EK, Quiocho FA, Mol Cell. 2007 Nov 30;28(4):614-23. PMID:18042456
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