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2qij
From Proteopedia
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(New page: 200px<br /><applet load="2qij" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qij, resolution 8.9Å" /> '''Hepatitis B Capsid Pr...)
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Revision as of 09:44, 23 January 2008
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Hepatitis B Capsid Protein with an N-terminal extension modelled into 8.9 A data.
Overview
Hepatitis B core (HBc) particles have been extensively exploited as, carriers for foreign immunological epitopes in the development of, multicomponent vaccines and diagnostic reagents. Crystals of the T = 4 HBc, particle were grown in PEG 20,000, ammonium sulfate and various types of, alcohols. A temperature jump from 277 or 283 to 290 K was found to enhance, crystal growth. A crystal grown using MPD as a cryoprotectant diffracted, X-rays to 7.7 A resolution and data were collected to 99.6% completeness, at 8.9 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell, parameters a = 352.3, b = 465.5, c = 645.0 A. The electron-density map, reveals a protrusion that is consistent with the N-terminus extending out, from the surface of the capsid. The structure presented here supports the, idea that N-terminal insertions can be exploited in the development of, diagnostic reagents, multicomponent vaccines and delivery vehicles into, mammalian cells.
About this Structure
2QIJ is a Single protein structure of sequence from Hepatitis b virus subtype adw. Full crystallographic information is available from OCA.
Reference
Crystallization and X-ray analysis of the T = 4 particle of hepatitis B capsid protein with an N-terminal extension., Tan WS, McNae IW, Ho KL, Walkinshaw MD, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Aug 1;63(Pt, 8):642-7. Epub 2007 Jul 21. PMID:17671358
Page seeded by OCA on Wed Jan 23 11:44:44 2008
