1obf
From Proteopedia
(New page: 200px<br /> <applet load="1obf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1obf, resolution 1.7Å" /> '''THE CRYSTAL STRUCTUR...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1OBF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ ]] with SO4, K and PG4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.12 1.2.1.12]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OBF OCA]]. | + | 1OBF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Achromobacter_xylosoxidans Achromobacter xylosoxidans]] with SO4, K and PG4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.12 1.2.1.12]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OBF OCA]]. |
==Reference== | ==Reference== | ||
The structure of glyceraldehyde 3-phosphate dehydrogenase from Alcaligenes xylosoxidans at 1.7 A resolution., Antonyuk SV, Eady RR, Strange RW, Hasnain SS, Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):835-42. Epub 2003, Apr 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12777799 12777799] | The structure of glyceraldehyde 3-phosphate dehydrogenase from Alcaligenes xylosoxidans at 1.7 A resolution., Antonyuk SV, Eady RR, Strange RW, Hasnain SS, Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):835-42. Epub 2003, Apr 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12777799 12777799] | ||
| + | [[Category: Achromobacter xylosoxidans]] | ||
| + | [[Category: Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Antonyuk, S.V.]] | [[Category: Antonyuk, S.V.]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:11:57 2007'' |
Revision as of 09:07, 30 October 2007
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THE CRYSTAL STRUCTURE OF GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE FROM ALCALIGENES XYLOSOXIDANS AT 1.7 RESOLUTION.
Overview
The enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the, Gram-negative denitrifying bacterial species Alcaligenes xylosoxidans was, purified and crystallized as a contaminant protein during purification of, nitrous oxide reductase. This is the first structure of a GAPDH from a, denitrifying species. The crystal structure was solved at 1.7 A resolution, by molecular replacement using the structure of GAPDH from Bacillus, stearothermophilus as a starting model. The quality of the structure, enabled the amino-acid sequence of the A. xylosoxidans GAPDH to be, assigned. The structure is that of the apo-enzyme, lacking the NAD+, cofactor and with the active-site residue Cys154 oxidized. The global, structure of the enzyme has a homotetrameric quaternary structure similar, to that ... [(full description)]
About this Structure
1OBF is a [Single protein] structure of sequence from [Achromobacter xylosoxidans] with SO4, K and PG4 as [ligands]. Active as [Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)], with EC number [1.2.1.12]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The structure of glyceraldehyde 3-phosphate dehydrogenase from Alcaligenes xylosoxidans at 1.7 A resolution., Antonyuk SV, Eady RR, Strange RW, Hasnain SS, Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):835-42. Epub 2003, Apr 25. PMID:12777799
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