2ego
From Proteopedia
(New page: 200px<br /><applet load="2ego" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ego, resolution 1.80Å" /> '''Crystal Structure of...) |
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| - | [[Image:2ego.jpg|left|200px]]<br /><applet load="2ego" size=" | + | [[Image:2ego.jpg|left|200px]]<br /><applet load="2ego" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2ego, resolution 1.80Å" /> | caption="2ego, resolution 1.80Å" /> | ||
'''Crystal Structure of Tamalin PDZ Domain'''<br /> | '''Crystal Structure of Tamalin PDZ Domain'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2EGO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http:// | + | 2EGO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EGO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ligand-free]] | [[Category: ligand-free]] | ||
[[Category: pdz domain]] | [[Category: pdz domain]] | ||
| + | [[Category: protein binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:49:19 2008'' |
Revision as of 09:49, 23 January 2008
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Crystal Structure of Tamalin PDZ Domain
Overview
Metabotropic glutamate receptors (mGluRs) function as neuronal, G-protein-coupled receptors and this requires efficient membrane targeting, through associations with cytoplasmic proteins. However, the molecular, mechanism regulating mGluR cell-surface trafficking remains unknown. We, report here that mGluR trafficking is controlled by the autoregulatory, assembly of a scaffold protein Tamalin. In the absence of mGluR, Tamalin, self-assembles into autoinhibited conformations, through its PDZ domain, and C-terminal intrinsic ligand motif. X-ray crystallographic analyses, visualized integral parts of the oligomeric self-assemblies of Tamalin, which require not only the novel hydrophobic dimerization interface but, also canonical and noncanonical PDZ/ligand autoinhibitory interactions., The mGluR cytoplasmic region can competitively bind to Tamalin at a higher, concentration, disrupting weak inhibitory interactions. The atomic view of, mGluR association suggests that this rearrangement is dominated by, electrostatic attraction and repulsion. We also observed in mammalian, cells that the association liberates the intrinsic ligand toward a motor, protein receptor, thereby facilitating mGluR cell-surface trafficking. Our, study suggests a novel regulatory mechanism of the PDZ domain, by which, Tamalin switches between the trafficking-inhibited and -active forms, depending on mGluR association.
About this Structure
2EGO is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Crystal structures of autoinhibitory PDZ domain of Tamalin: implications for metabotropic glutamate receptor trafficking regulation., Sugi T, Oyama T, Muto T, Nakanishi S, Morikawa K, Jingami H, EMBO J. 2007 Apr 18;26(8):2192-2205. Epub 2007 Mar 29. PMID:17396155
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