Beta-glucosidase

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A [[β-glucosidase]] is an '''enzyme''' which catalyses the hydrolysis of terminal non-reducing residues in β-glucosides (EC number : 3.2.1.21). In the case of 2VRJ, it comes from ''Thermotoga maritima'' which is a rod-shaped bacterium belonging to the order of Thermotogates. This bacterium was originally isolated from geothermal heated marine sediments.
A [[β-glucosidase]] is an '''enzyme''' which catalyses the hydrolysis of terminal non-reducing residues in β-glucosides (EC number : 3.2.1.21). In the case of 2VRJ, it comes from ''Thermotoga maritima'' which is a rod-shaped bacterium belonging to the order of Thermotogates. This bacterium was originally isolated from geothermal heated marine sediments.
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2VRJ is here is in complex with an inhibitor called N-octyl-5-deoxy66-oxa-N-carbamoylcalystegine [1].
+
2VRJ is here is in complex with an inhibitor called N-octyl-5-deoxy66-oxa-N-carbamoylcalystegine <ref>PMID: 18833549</ref>.
===General action as biocatalyst===
===General action as biocatalyst===
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It acts on the '''β(1-4) bond linking''' two glucose residues or glucose-substituted molecules. The action of the enzyme on such glucosides results in the release of units of glucose. For instance, hydrolysis of cellobiose catalysed by a β-glucosidase releases two glucoses [2].
+
It acts on the '''β(1-4) bond linking''' two glucose residues or glucose-substituted molecules. The action of the enzyme on such glucosides results in the release of units of glucose. For instance, hydrolysis of cellobiose catalysed by a β-glucosidase releases two glucoses <ref>http://en.wikipedia.org/wiki/B-glucosidase</ref>.
[[ Image:Cellobiose.jpg]][[Image:Suite.jpg]]
[[ Image:Cellobiose.jpg]][[Image:Suite.jpg]]
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In terms of structure 2VRJ is a homodimer. It means that it is composed of two chains <scene name='Sandbox_155/Chain_a/1'>A</scene> and <scene name='Sandbox_155/Chain_b/1'>B</scene> which are chiral. Each chain is composed of 438 residues and constitutes a subunit of the protein. Each subunit contains a''' catalytic site'''.
In terms of structure 2VRJ is a homodimer. It means that it is composed of two chains <scene name='Sandbox_155/Chain_a/1'>A</scene> and <scene name='Sandbox_155/Chain_b/1'>B</scene> which are chiral. Each chain is composed of 438 residues and constitutes a subunit of the protein. Each subunit contains a''' catalytic site'''.
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The enzymatic hydrolysis of a glycosidic bond requires two critical residues : a proton donor and a proton acceptor which can also be called a nucleophile/base. Aspartate and glutamate have been found to perform catalysis [3]. Accorded to this, studies showed that one of the conserved regions of β-glucosidases is centred on conserved glutamate residues [4].
+
The enzymatic hydrolysis of a glycosidic bond requires two critical residues : a proton donor and a proton acceptor which can also be called a nucleophile/base. Aspartate and glutamate have been found to perform catalysis <ref>PMID: 8535779</ref>. Accorded to this, studies showed that one of the conserved regions of β-glucosidases is centred on conserved glutamate residues <ref>http://www.ebi.ac.uk/interpro/IEntry?ac=IPR018120#PUB00002205</ref>.
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As every β-glucosidase, 2VRJ presents two conserved residues of glutamate (<scene name='Sandbox_155/166_and_351/4'>166 and 351</scene>). Moreover 2VRJ has a third important residue : <scene name='Sandbox_155/293/2'>asparagin 293</scene> [5].
+
As every β-glucosidase, 2VRJ presents two conserved residues of glutamate (<scene name='Sandbox_155/166_and_351/4'>166 and 351</scene>). Moreover 2VRJ has a third important residue : <scene name='Sandbox_155/293/2'>asparagin 293</scene> <ref>http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/CSA/CSA_Site_Wrapper.pl?pdb=2vrj</ref>.
The protein is presented in complex with an inhibitor called <scene name='Sandbox_155/Calystegine/1'>calystegine</scene>. We can see that the two '''<font color='#5CB8D1'>glutamate</font>''' residues and the asparagin are really closed to each other and to the <scene name='Sandbox_155/Ligand_and_residues/1'>ligand</scene>. Such a proximity highly suggests that there are important interactions between them. So we can say that the catalytic site of 2VRJ is composed of two glutamate and one asparagin.
The protein is presented in complex with an inhibitor called <scene name='Sandbox_155/Calystegine/1'>calystegine</scene>. We can see that the two '''<font color='#5CB8D1'>glutamate</font>''' residues and the asparagin are really closed to each other and to the <scene name='Sandbox_155/Ligand_and_residues/1'>ligand</scene>. Such a proximity highly suggests that there are important interactions between them. So we can say that the catalytic site of 2VRJ is composed of two glutamate and one asparagin.
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There are three different topologies for the active site of β-glucosidases : the pocket or crater, the cleft or groove and the tunnel [3]. The topology of 2VRJ active site is a <scene name='Sandbox_155/Pocket/3'>pocket</scene> in which the ligand can bind.
+
There are three different topologies for the active site of β-glucosidases : the pocket or crater, the cleft or groove and the tunnel <ref>PMID: 8535779</ref>. The topology of 2VRJ active site is a <scene name='Sandbox_155/Pocket/3'>pocket</scene> in which the ligand can bind.
===Hydrolysis of terminal non-reducing residues in β-glucosides===
===Hydrolysis of terminal non-reducing residues in β-glucosides===
-
There are two ways to hydrolyse the terminal non-reducing residues in β-glucosides which implicate the two glutamate residues and a molecule of water [6]. Water which is an amphoter, is here used as a base for the nucleophilic attack on the positively charged anomeric carbon.
+
There are two ways to hydrolyse the terminal non-reducing residues in β-glucosides which implicate the two glutamate residues and a molecule of water <ref>http://www.cazy.org/fam/ghf_INV_RET.html#3</ref>. Water which is an amphoter, is here used as a base for the nucleophilic attack on the positively charged anomeric carbon.
The general equation of the chemical reaction is :
The general equation of the chemical reaction is :
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== References ==
== References ==
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+
<references/>
-
[1] Aguilar M, Gloster T M, García-Moreno I M, Ortiz Mellet C, Davies G J, Llebaria A, Casas J, Egido-Gabás M, García Fernandez J M. Molecular Basis for β-Glucosidase Inhibition by Ring-Modified Calystegine Analogues. ChemBioChem 2008 ; 9 (16) : 2612-8.
+
[1] Aguilar M, Gloster T M, García-Moreno I M, Ortiz Mellet C, Davies G J, Llebaria A, Casas J, Egido-Gabás M, García Fernandez J M. Molecular Basis for β-Glucosidase Inhibition by Ring-Modified Calystegine Analogues. ChemBioChem 2008 ; 9 (16) : 2612-8. PMID: 18833549
[2] http://en.wikipedia.org/wiki/B-glucosidase
[2] http://en.wikipedia.org/wiki/B-glucosidase
-
[3] Davies G, Henrissat B. Structures and mechanisms of glycosyl hydrolases. Structure 2004 ; 3 (9) : 853-9.
+
[3] Davies G, Henrissat B. Structures and mechanisms of glycosyl hydrolases. Structure 2004 ; 3 (9) : 853-9. PMID: 8535779
[4] http://www.ebi.ac.uk/interpro/IEntry?ac=IPR018120#PUB00002205
[4] http://www.ebi.ac.uk/interpro/IEntry?ac=IPR018120#PUB00002205

Revision as of 09:53, 26 November 2010

Template:STRUCTURE 2vrj

A β-glucosidase is an enzyme which catalyses the hydrolysis of terminal non-reducing residues in β-glucosides (EC number : 3.2.1.21). In the case of 2VRJ, it comes from Thermotoga maritima which is a rod-shaped bacterium belonging to the order of Thermotogates. This bacterium was originally isolated from geothermal heated marine sediments. 2VRJ is here is in complex with an inhibitor called N-octyl-5-deoxy66-oxa-N-carbamoylcalystegine [1].


Contents

General action as biocatalyst

It acts on the β(1-4) bond linking two glucose residues or glucose-substituted molecules. The action of the enzyme on such glucosides results in the release of units of glucose. For instance, hydrolysis of cellobiose catalysed by a β-glucosidase releases two glucoses [2].

Image:Cellobiose.jpgImage:Suite.jpg

β-glucosidases can also be called β-D-glucoside glucohydrolases or cellobiases.


2VRJ

Structure and function

In terms of structure 2VRJ is a homodimer. It means that it is composed of two chains and which are chiral. Each chain is composed of 438 residues and constitutes a subunit of the protein. Each subunit contains a catalytic site. The enzymatic hydrolysis of a glycosidic bond requires two critical residues : a proton donor and a proton acceptor which can also be called a nucleophile/base. Aspartate and glutamate have been found to perform catalysis [3]. Accorded to this, studies showed that one of the conserved regions of β-glucosidases is centred on conserved glutamate residues [4]. As every β-glucosidase, 2VRJ presents two conserved residues of glutamate (). Moreover 2VRJ has a third important residue : [5]. The protein is presented in complex with an inhibitor called . We can see that the two glutamate residues and the asparagin are really closed to each other and to the . Such a proximity highly suggests that there are important interactions between them. So we can say that the catalytic site of 2VRJ is composed of two glutamate and one asparagin.

There are three different topologies for the active site of β-glucosidases : the pocket or crater, the cleft or groove and the tunnel [6]. The topology of 2VRJ active site is a in which the ligand can bind.

Hydrolysis of terminal non-reducing residues in β-glucosides

There are two ways to hydrolyse the terminal non-reducing residues in β-glucosides which implicate the two glutamate residues and a molecule of water [7]. Water which is an amphoter, is here used as a base for the nucleophilic attack on the positively charged anomeric carbon.

The general equation of the chemical reaction is : Image:Jpp.jpg

Inverting glycoside hydrolases

Inverting glycoside hydrolases lead to an inversion of the anomeric configuration to create an alpha configuration. The steps of the reaction are like the mechanism of nucleophilic substitution S2N. It is an one step process: the nucleophile (water) the anomeric carbon with simultaneous expulsion of the leaving group (OR). Bond making takes place at the same time as bond breaking. Such a mechanism is called concerted reaction. The distance between the two carboxylates is about 10.5 angströms.


Retaining glycoside hydrolases

Retaining glycoside hydrolyses occur in two steps: the first step, called glycosylation leads to the release of the leaving group and the creation of a carbocation. Subsequently, water attacks this last one. The second step, called deglycosylation consists of OR- nucleophilic attack on the intermediate and allows the deglycosylation of the enzyme. In this case, there are two transition states involved. The distance between the two carboxylates for this mechanism is about 5.5 angströms. For 2VRJ the distance between its two glutamates is about angströms. So we can say that 2VRJ seems to be a retaining enzyme.


NB: The values of the pH and the nature of the solvent play a main role in the rate of the reaction.

Glutamates are directly involved in the catalytic reaction but asparagine is used to stabilise the structure.

Other use of β-glucosidases

β-glucosidase is now used for the synthesis of biofuel. Wood is an abundant and renewable energy which can be changed into bioethanol thanks to enzymatic hydrolysis. This synthesis needs five steps. First it is pre-hydrolysis. The structure is divided into lignin and (hemi)cellulose. The cellulase can better access the structure to act on it. The second step: hydrolysis is the most important. A cellulase is a complex of 3 enzymes which act together to hydrolyse cellulose: Endoglucanase breaks the chain in the middle of the molecular structure of cellulose. Exoglucanase binds an available end of the chain and isolates it. Then units of cellobiose are cut (two units of glucose which are together). To finish, β-glucosidase divides cellobiose into two glucoses. When they ferment, they become ethanol. The final product is obtained thanks to fermentation, distillation and deshydratation.

Additional Resources

For additional information, see: Carbohydrate Metabolism

References

  1. Aguilar M, Gloster TM, Garcia-Moreno MI, Ortiz Mellet C, Davies GJ, Llebaria A, Casas J, Egido-Gabas M, Garcia Fernandez JM. Molecular basis for beta-glucosidase inhibition by ring-modified calystegine analogues. Chembiochem. 2008 Nov 3;9(16):2612-8. PMID:18833549 doi:10.1002/cbic.200800451
  2. http://en.wikipedia.org/wiki/B-glucosidase
  3. Davies G, Henrissat B. Structures and mechanisms of glycosyl hydrolases. Structure. 1995 Sep 15;3(9):853-9. PMID:8535779
  4. http://www.ebi.ac.uk/interpro/IEntry?ac=IPR018120#PUB00002205
  5. http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/CSA/CSA_Site_Wrapper.pl?pdb=2vrj
  6. Davies G, Henrissat B. Structures and mechanisms of glycosyl hydrolases. Structure. 1995 Sep 15;3(9):853-9. PMID:8535779
  7. http://www.cazy.org/fam/ghf_INV_RET.html#3

[1] Aguilar M, Gloster T M, García-Moreno I M, Ortiz Mellet C, Davies G J, Llebaria A, Casas J, Egido-Gabás M, García Fernandez J M. Molecular Basis for β-Glucosidase Inhibition by Ring-Modified Calystegine Analogues. ChemBioChem 2008 ; 9 (16) : 2612-8. PMID: 18833549

[2] http://en.wikipedia.org/wiki/B-glucosidase

[3] Davies G, Henrissat B. Structures and mechanisms of glycosyl hydrolases. Structure 2004 ; 3 (9) : 853-9. PMID: 8535779

[4] http://www.ebi.ac.uk/interpro/IEntry?ac=IPR018120#PUB00002205

[5] http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/CSA/CSA_Site_Wrapper.pl?pdb=2vrj

[6] http://www.cazy.org/fam/ghf_INV_RET.html#3

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