2v8k
From Proteopedia
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| - | [[Image:2v8k.jpg|left|200px]]<br /><applet load="2v8k" size=" | + | [[Image:2v8k.jpg|left|200px]]<br /><applet load="2v8k" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2v8k, resolution 2.1Å" /> | caption="2v8k, resolution 2.1Å" /> | ||
'''STRUCTURE OF A FAMILY 2 PECTATE LYASE IN COMPLEX WITH TRIGALACTURONIC ACID'''<br /> | '''STRUCTURE OF A FAMILY 2 PECTATE LYASE IN COMPLEX WITH TRIGALACTURONIC ACID'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2V8K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica] with TGU as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pectate_lyase Pectate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.2 4.2.2.2] Known structural/functional Site: <scene name='pdbsite=AC1:Tgu Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2V8K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica] with <scene name='pdbligand=TGU:'>TGU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pectate_lyase Pectate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.2 4.2.2.2] Known structural/functional Site: <scene name='pdbsite=AC1:Tgu Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V8K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: periplasm]] | [[Category: periplasm]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:50:23 2008'' |
Revision as of 09:50, 23 January 2008
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STRUCTURE OF A FAMILY 2 PECTATE LYASE IN COMPLEX WITH TRIGALACTURONIC ACID
Overview
The family 2 pectate lyase from Yersinia enterocolitica (YePL2A), solved, to 1.5A(,) reveals it to be the first prokaryotic protein reported to, display the rare (alpha/alpha)(7) barrel fold. In addition to its apo, form, we have also determined the structure of a metal-bound form of, YePL2A (to 2.0A()) and a trigalacturonic acid-bound substrate complex (to, 2.1A().) Although its fold is rare, the catalytic center of YePL2A can be, superimposed with structurally unrelated families, underlining the, conserved catalytic amino acid architecture of the beta-elimination, mechanism. In addition to its overall structure, YePL2A also has two other, unique features: 1) it utilizes a metal atom other than calcium for, catalysis, and 2) its Bronstead base is in an alternate conformation and, directly interacts with the uronate group of the substrate.
About this Structure
2V8K is a Single protein structure of sequence from Yersinia enterocolitica with as ligand. Active as Pectate lyase, with EC number 4.2.2.2 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
A Family 2 Pectate Lyase Displays a Rare Fold and Transition Metal-assisted -Elimination., Abbott DW, Boraston AB, J Biol Chem. 2007 Nov 30;282(48):35328-36. Epub 2007 Sep 19. PMID:17881361
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