1ea0
From Proteopedia
(New page: 200px<br /> <applet load="1ea0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ea0, resolution 3.0Å" /> '''ALPHA SUBUNIT OF A. ...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1EA0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Azospirillum_brasilense Azospirillum brasilense]] with OMT, FMN, AKG and F3S as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.1.13 1.4.1.13]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EA0 OCA]]. | + | 1EA0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Azospirillum_brasilense Azospirillum brasilense]] with OMT, FMN, AKG and F3S as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Glutamate_synthase_(NADPH) Glutamate synthase (NADPH)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.1.13 1.4.1.13]]. Structure known Active Site: OMA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EA0 OCA]]. |
==Reference== | ==Reference== | ||
Cross-talk and ammonia channeling between active centers in the unexpected domain arrangement of glutamate synthase., Binda C, Bossi RT, Wakatsuki S, Arzt S, Coda A, Curti B, Vanoni MA, Mattevi A, Structure. 2000 Dec 15;8(12):1299-308. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11188694 11188694] | Cross-talk and ammonia channeling between active centers in the unexpected domain arrangement of glutamate synthase., Binda C, Bossi RT, Wakatsuki S, Arzt S, Coda A, Curti B, Vanoni MA, Mattevi A, Structure. 2000 Dec 15;8(12):1299-308. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11188694 11188694] | ||
[[Category: Azospirillum brasilense]] | [[Category: Azospirillum brasilense]] | ||
| + | [[Category: Glutamate synthase (NADPH)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Binda, C.]] | [[Category: Binda, C.]] | ||
| Line 24: | Line 25: | ||
[[Category: complex iron sulphur flavoprotein]] | [[Category: complex iron sulphur flavoprotein]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:12:52 2007'' |
Revision as of 09:08, 30 October 2007
|
ALPHA SUBUNIT OF A. BRASILENSE GLUTAMATE SYNTHASE
Overview
INTRODUCTION: The complex iron-sulfur flavoprotein glutamate synthase, catalyses the reductive synthesis of L-glutamate from 2-oxoglutarate and, L-glutamine, a reaction in the plant and bacterial pathway for ammonia, assimilation. The enzyme functions through three distinct active centers, carrying out L-glutamine hydrolysis, conversion of 2-oxoglutarate into, L-glutamate, and electron uptake from an electron donor. RESULTS: The 3.0, A crystal structure of the dimeric 324 kDa core protein of a bacterial, glutamate synthase was solved by the MAD method, using the very weak, anomalous signal of the two 3Fe-4S clusters present in the asymmetric, unit. The 1,472 amino acids of the monomer fold into a four-domain, architecture. The two catalytic domains have canonical, Ntn-amidotransferase and ... [(full description)]
About this Structure
1EA0 is a [Single protein] structure of sequence from [Azospirillum brasilense] with OMT, FMN, AKG and F3S as [ligands]. Active as [Glutamate synthase (NADPH)], with EC number [1.4.1.13]. Structure known Active Site: OMA. Full crystallographic information is available from [OCA].
Reference
Cross-talk and ammonia channeling between active centers in the unexpected domain arrangement of glutamate synthase., Binda C, Bossi RT, Wakatsuki S, Arzt S, Coda A, Curti B, Vanoni MA, Mattevi A, Structure. 2000 Dec 15;8(12):1299-308. PMID:11188694
Page seeded by OCA on Tue Oct 30 11:12:52 2007
