This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2qrl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search

OCA (Talk | contribs)
(New page: 200px<br /><applet load="2qrl" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qrl, resolution 1.600&Aring;" /> '''Crystal Structure o...)
Next diff →

Revision as of 09:57, 23 January 2008

Image:2qrl.gif

2qrl, resolution 1.600Å

Drag the structure with the mouse to rotate

Crystal Structure of Oxalylglycine-bound Saccharopine Dehydrogenase (L-Lys Forming) from Saccharomyces cerevisiae

Overview

Three structures of saccharopine dehydrogenase (l-lysine-forming) (SDH), have been determined in the presence of sulfate, adenosine monophosphate, (AMP), and oxalylglycine (OxGly). In the sulfate-bound structure, a, sulfate ion binds in a cleft between the two domains of SDH, occupies one, of the substrate carboxylate binding sites, and results in partial closure, of the active site of the enzyme due to a domain rotation of almost 12, degrees in comparison to the apoenzyme structure. In the second structure, AMP binds to the active site in an area where the NAD+ cofactor is, expected to bind. All of the AMP moieties (adenine ring, ribose, and, phosphate) interact with specific residues of the enzyme. In the, OxGly-bound structure, carboxylates of OxGly interact with arginine, residues representative of the manner in which substrate, (alpha-ketoglutarate and saccharopine) may bind. The alpha-keto group of, OxGly interacts with Lys77 and His96, which are candidates for acid-base, catalysis. Analysis of ligand-enzyme interactions, comparative structural, analysis, corroboration with kinetic data, and discussion of a ternary, complex model are presented in this study.

About this Structure

2QRL is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Active as Saccharopine dehydrogenase (NAD(+), L-lysine-forming), with EC number 1.5.1.7 Full crystallographic information is available from OCA.

Reference

Crystal Structures of Ligand-Bound Saccharopine Dehydrogenase from Saccharomyces cerevisiae(,)., Andi B, Xu H, Cook PF, West AH, Biochemistry. 2007 Nov 6;46(44):12512-21. Epub 2007 Oct 16. PMID:17939687

Page seeded by OCA on Wed Jan 23 11:57:29 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2qrl"
Personal tools