2pll
From Proteopedia
(New page: 200px<br /> <applet load="2pll" size="450" color="white" frame="true" align="right" spinBox="true" caption="2pll, resolution 1.90Å" /> '''Crystal structure o...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2pll. | + | [[Image:2pll.jpg|left|200px]]<br /><applet load="2pll" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2pll" size=" | + | |
caption="2pll, resolution 1.90Å" /> | caption="2pll, resolution 1.90Å" /> | ||
'''Crystal structure of perdeuterated human arginase I'''<br /> | '''Crystal structure of perdeuterated human arginase I'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2PLL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MN and ABH as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Arginase Arginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.1 3.5.3.1] Full crystallographic information is available from [http:// | + | 2PLL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=ABH:'>ABH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Arginase Arginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.1 3.5.3.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PLL OCA]. |
==Reference== | ==Reference== | ||
| Line 25: | Line 24: | ||
[[Category: perdeuterated protein; x-ray structure]] | [[Category: perdeuterated protein; x-ray structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:04:59 2008'' |
Revision as of 10:04, 23 January 2008
|
Crystal structure of perdeuterated human arginase I
Overview
Arginase is a manganese metalloenzyme that catalyzes the hydrolysis of, l-arginine to yield l-ornithine and urea. In order to establish a, foundation for future neutron diffraction studies that will provide, conclusive structural information regarding proton/deuteron positions in, enzyme-inhibitor complexes, we have expressed, purified, assayed, and, determined the X-ray crystal structure of perdeuterated (i.e., fully, deuterated) human arginase I complexed with 2(S)-amino-6-boronohexanoic, acid (ABH) at 1.90A resolution. Prior to the neutron diffraction, experiment, it is important to establish that perdeuteration does not, cause any unanticipated structural or functional changes. Accordingly, we, find that perdeuterated human arginase I exhibits catalytic activity, essentially identical to that of the unlabeled enzyme. Additionally, the, structure of the perdeuterated human arginase I-ABH complex is identical, to that of the corresponding complex with the unlabeled enzyme. Therefore, we conclude that crystals of the perdeuterated human arginase I-ABH, complex are suitable for neutron crystallographic study.
About this Structure
2PLL is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Arginase, with EC number 3.5.3.1 Full crystallographic information is available from OCA.
Reference
Expression, purification, assay, and crystal structure of perdeuterated human arginase I., Di Costanzo L, Moulin M, Haertlein M, Meilleur F, Christianson DW, Arch Biochem Biophys. 2007 May 21;. PMID:17562323
Page seeded by OCA on Wed Jan 23 12:04:59 2008
