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Heat Shock Proteins

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[[Image:3los.jpg|left|200px|thumb|Crystal Structure of Hsp60 [[3los]]]]
[[Image:3los.jpg|left|200px|thumb|Crystal Structure of Hsp60 [[3los]]]]
{{STRUCTURE_3los| PDB=3los | SIZE=300| SCENE= |right|CAPTION=Hsp60 [[3los]] }}
{{STRUCTURE_3los| PDB=3los | SIZE=300| SCENE= |right|CAPTION=Hsp60 [[3los]] }}
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[[Heat Shock Proteins]] (Hsp). The Hsp are proteins which are expressed when cells are exposed to stresses like heat. Hsp60, Hsp70, Hsp90 etc. are named according to their molecular weights. Their function is to provide protection from stress. The images at the left and at the right correspond to one representative Hsp, ''i.e.'' the crystal structure of Hsp60 from ''Methanococcus maripaludis '' ([[3los]]).
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{{TOC limit|limit=2}}
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== 3D Structures of Heat Shock Proteins ==
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=== Hsp60 - the large subunit of the chaperonin molecule GroEL ===
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[[3los]] – MmHsp60 - GroEL – Methanococcus maripaludis – closed state
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3iyf - MmHsp60 - GroEL – open state
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3e76 – EcHsp60 – GroEL+Tl ion – Escherichia coli
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2nwc, 1oel, 1grl - EcHsp60 – GroEL
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2eu1 - EcHsp60 (mutant) – GroEL
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2cgt - EcHsp60 – GroEL+ADP+capsid assembly protein Gp31
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2c7c, 2c7d - EcHsp60 – GroEL+GroES – cryo EM
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1we3, 1wf4 - TtHsp60 – GroEL+GroES – Thermus thermophilus
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1gr5 - EcHsp60 – GroEL – cryo EM
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1gru - EcHsp60 – GroEL+ATP+GroES+ADP – cryo EM
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1dkd - EcHsp60 – GroEL apical (top) domain (146 amino acids)+12-mer peptide
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1dk7, 1jon - EcHsp60 – GroEL apical domain
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1srv - TtHsp60 – GroEL apical domain
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1ass, 1asx - Hsp60 – GroEL apical domain – Thermoplasma acidophilum
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1kid - EcHsp60 (mutant) – GroEL apical domain
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=== Hsp70 – 3 domains: N-terminus ATPase hydrolyzes ATP to ADP (NBD); hydrophobic amino acid binding domain (sub): C-terminus lid domain (lid). ===
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3a8y – hHsp70 NBD+BAG domain regulator protein – human
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3fzf - hHsp70 NBD+BAG domain regulator protein+ATP
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3fzh, 3fzm - hHsp70 NBD+BAG domain regulator protein+ adenosine derivative
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3fzk, 3fzl - hHsp70 NBD+BAG domain regulator protein+ purine derivative
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3cqx - Hsp70 NBD+BAG domain regulator protein – mouse
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3lof – hHsp70 lid
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2p32 - CeHsp70 lid
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3l6q - CpHsp70 NBD – Cryptosporidium parvum
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3kvg - CpHsp70 NBD+AMPPNP
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3l4i - CpHsp70 NBD+ADP+P
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3jxu, 3i33, 3gdq, 3fe1 - hHsp70 NBD+ADP+P
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3iuc - hHsp70 NBD+ADP
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2e88, 1s3x, 1hjo - hHsp70 NBD
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2e8a - hHsp70 NBD+AMPPNP
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2qw9 – cHsp70 NBD – cow
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2bup - cHsp70 NBD (mutant)
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2qwl, 2qwm - cHsp70 NBD+ADP
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2qwn, 2qwo, 2qwp, 2qwq, 2qwr - cHsp70 NBD (mutant)+ auxilin protein
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2kho – EcHsp70 – Escherichia coli – NMR
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3gl1 - ScHsp70 NBD – Saccharomyces cerevisiae
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3dpo – EcHsp70 sub+12-mer peptide
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3dpp, 3dpq – EcHsp70 sub+20-mer peptide
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3dob, 3dqg, 2op6 - CeHsp70 sub – Caenoharbditis elegans
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2d2e, 3d2f – ScHsp70 NBD+SSE1(Hsp110)
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2v7z – rHsp70 NBD+sub – rat
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7hsc, 1ckr - rHsp70 sub - NMR
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=== Hsp90 – 4 domains: N-terminus (NTD); linker; middle (MD); C-terminus (CT) ===
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3k97, 3hek– hHsp90 NTD+pyrrolidin derivative – human
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3k98, 3k99 - hHsp90 NTD+indole derivative
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3ied - Hsp90 NTD+AMPPN – Plasmodium falciparum
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2wi1, 2wi2, 2wi3, 2wi4, 2wi5, 2wi6, 2wi7 - hHsp90 NTD+2-amino thienopyrimidine
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3hhu - hHsp90 NTD+ carbamic acid derivative
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3ft8, 3ft5 - hHsp90 NTD+quinazoline derivative
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2k5b - hHsp90 NTD+Cdc37 – NMR - docking
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3eko, 3ekr - hHsp90 NTD+dihydroxyphenyl amide
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2jki - Hsp90 NTD+SGT1 domain protein –barley
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2jjc - hHsp90 NTD+pyrimidine-2-amine
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3bm9, 3bmy - hHsp90 NTD+benzisoxazole
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2of6 - hHsp90 NTD+triazine derivative
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2qfo - hHsp90 NTD+pyrimidine derivative
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1uy6, 1uy7, 1uy8, 1uy9, 1uyc, 1uyd, 1uye, 1uyf, 1uyg, 1uyh, 1uyi, 1uyk, 1uyl - hHsp90 NTD+purine derivative
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2qg0, 2bz5 - hHsp90 NTD+sulfonamide derivative
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2qg2, 2cvi, 2cvj, 2uwd - hHsp90 NTD+oxazol derivative
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3d0b - hHsp90 NTD+benzamide derivative
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2fwy, 2fwz, 2h55, 1uym - hHsp90 NTD+PU inhibitor
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2ccs, 2cct, 2ccu, 2cdd, 2byh, 2byi, 2bsm, 2bt0, 1yc3 - hHsp90 NTD+pyrazol derivative
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2brc, 2bre, 1am1 - ScHsp90 NTD+pyrazol derivative
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1amw - ScHsp90 NTD+ADP
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1ah6, 1ah8 - ScHsp90 NTD
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1osf, 1yer, 1yes, 1yet - hHsp90 NTD+geldamycin
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1bgq – yHsp90+radicicol - yeast
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1byq - hHsp90 NTD+ATP-Mg
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1a4h – yHsp90+ geldamycin
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=== Hsp82 is the yeast equivalent of Hsp90 in mammals ===
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1ah6, 1ah8 – yHsp82 NTD
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2wep – yHsp82 NTD (mutant)+ADP
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2akp - yHsp82 NTD (mutant)
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1weq, 3c11 - yHsp82 NTD (mutant)+ geldamycin
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2wer - yHsp82 NTD (mutant)+radicicol
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1bgq - yHsp82 NTD +radicicol
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2iws, 2iwu, 2iwx, 2cgf - yHsp82 NTD +radicicol analogues
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2vw5, 2vwc - yHsp82 NTD +7-o-carbamoylpremacbecin
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3c0e - yHsp82 NTD (mutant)
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2fxs - yHsp82 NTD +radamide
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1zw9 - yHsp82 NTD + adenine derivative
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1zwh - yHsp82 NTD + radester amine
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2brc, 2bre - yHsp82 NTD+pyrazol derivative
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2cg9 – yHsp82+co-chaperone SBA1
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2cge - yHsp82 MD+CT+co-chaperone SBA1
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1hk7 - yHsp82 MD
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1usu, 1usv - yHsp82 MD+co-chaperone AHA1
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1us7 - yHsp82 NTD+co-chaperone cdc37
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=== Hsp100 are named ClpA or ClpB ===
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1r6b, 1ksf – EcClpA (mutant) - Escherichia coli
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1khy – EcClpB NTD
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1jbk – EcClpB NBD
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1k6k - EcClpA NTD
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=== Hsp110 in mammals is named Sse1, Sse2 in yeast ===
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3d2e, 3d2f, 3c7n – Sse1+Hsp70 – yeast
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2qxl – Sse1 - yeast

Revision as of 09:24, 15 December 2010

Image:3los.jpg
Crystal Structure of Hsp60 3los

Template:STRUCTURE 3los

Heat Shock Proteins (Hsp). The Hsp are proteins which are expressed when cells are exposed to stresses like heat. Hsp60, Hsp70, Hsp90 etc. are named according to their molecular weights. Their function is to provide protection from stress. The images at the left and at the right correspond to one representative Hsp, i.e. the crystal structure of Hsp60 from Methanococcus maripaludis (3los).

Contents

3D Structures of Heat Shock Proteins

Hsp60 - the large subunit of the chaperonin molecule GroEL

3los – MmHsp60 - GroEL – Methanococcus maripaludis – closed state 3iyf - MmHsp60 - GroEL – open state 3e76 – EcHsp60 – GroEL+Tl ion – Escherichia coli 2nwc, 1oel, 1grl - EcHsp60 – GroEL 2eu1 - EcHsp60 (mutant) – GroEL 2cgt - EcHsp60 – GroEL+ADP+capsid assembly protein Gp31 2c7c, 2c7d - EcHsp60 – GroEL+GroES – cryo EM 1we3, 1wf4 - TtHsp60 – GroEL+GroES – Thermus thermophilus 1gr5 - EcHsp60 – GroEL – cryo EM 1gru - EcHsp60 – GroEL+ATP+GroES+ADP – cryo EM 1dkd - EcHsp60 – GroEL apical (top) domain (146 amino acids)+12-mer peptide 1dk7, 1jon - EcHsp60 – GroEL apical domain 1srv - TtHsp60 – GroEL apical domain 1ass, 1asx - Hsp60 – GroEL apical domain – Thermoplasma acidophilum 1kid - EcHsp60 (mutant) – GroEL apical domain


Hsp70 – 3 domains: N-terminus ATPase hydrolyzes ATP to ADP (NBD); hydrophobic amino acid binding domain (sub): C-terminus lid domain (lid).

3a8y – hHsp70 NBD+BAG domain regulator protein – human 3fzf - hHsp70 NBD+BAG domain regulator protein+ATP 3fzh, 3fzm - hHsp70 NBD+BAG domain regulator protein+ adenosine derivative 3fzk, 3fzl - hHsp70 NBD+BAG domain regulator protein+ purine derivative 3cqx - Hsp70 NBD+BAG domain regulator protein – mouse 3lof – hHsp70 lid 2p32 - CeHsp70 lid 3l6q - CpHsp70 NBD – Cryptosporidium parvum 3kvg - CpHsp70 NBD+AMPPNP 3l4i - CpHsp70 NBD+ADP+P 3jxu, 3i33, 3gdq, 3fe1 - hHsp70 NBD+ADP+P 3iuc - hHsp70 NBD+ADP 2e88, 1s3x, 1hjo - hHsp70 NBD 2e8a - hHsp70 NBD+AMPPNP 2qw9 – cHsp70 NBD – cow 2bup - cHsp70 NBD (mutant) 2qwl, 2qwm - cHsp70 NBD+ADP 2qwn, 2qwo, 2qwp, 2qwq, 2qwr - cHsp70 NBD (mutant)+ auxilin protein 2kho – EcHsp70 – Escherichia coli – NMR 3gl1 - ScHsp70 NBD – Saccharomyces cerevisiae 3dpo – EcHsp70 sub+12-mer peptide 3dpp, 3dpq – EcHsp70 sub+20-mer peptide 3dob, 3dqg, 2op6 - CeHsp70 sub – Caenoharbditis elegans 2d2e, 3d2f – ScHsp70 NBD+SSE1(Hsp110) 2v7z – rHsp70 NBD+sub – rat 7hsc, 1ckr - rHsp70 sub - NMR


Hsp90 – 4 domains: N-terminus (NTD); linker; middle (MD); C-terminus (CT)

3k97, 3hek– hHsp90 NTD+pyrrolidin derivative – human 3k98, 3k99 - hHsp90 NTD+indole derivative 3ied - Hsp90 NTD+AMPPN – Plasmodium falciparum 2wi1, 2wi2, 2wi3, 2wi4, 2wi5, 2wi6, 2wi7 - hHsp90 NTD+2-amino thienopyrimidine 3hhu - hHsp90 NTD+ carbamic acid derivative 3ft8, 3ft5 - hHsp90 NTD+quinazoline derivative 2k5b - hHsp90 NTD+Cdc37 – NMR - docking 3eko, 3ekr - hHsp90 NTD+dihydroxyphenyl amide 2jki - Hsp90 NTD+SGT1 domain protein –barley 2jjc - hHsp90 NTD+pyrimidine-2-amine 3bm9, 3bmy - hHsp90 NTD+benzisoxazole 2of6 - hHsp90 NTD+triazine derivative 2qfo - hHsp90 NTD+pyrimidine derivative 1uy6, 1uy7, 1uy8, 1uy9, 1uyc, 1uyd, 1uye, 1uyf, 1uyg, 1uyh, 1uyi, 1uyk, 1uyl - hHsp90 NTD+purine derivative 2qg0, 2bz5 - hHsp90 NTD+sulfonamide derivative 2qg2, 2cvi, 2cvj, 2uwd - hHsp90 NTD+oxazol derivative 3d0b - hHsp90 NTD+benzamide derivative 2fwy, 2fwz, 2h55, 1uym - hHsp90 NTD+PU inhibitor 2ccs, 2cct, 2ccu, 2cdd, 2byh, 2byi, 2bsm, 2bt0, 1yc3 - hHsp90 NTD+pyrazol derivative 2brc, 2bre, 1am1 - ScHsp90 NTD+pyrazol derivative 1amw - ScHsp90 NTD+ADP 1ah6, 1ah8 - ScHsp90 NTD 1osf, 1yer, 1yes, 1yet - hHsp90 NTD+geldamycin 1bgq – yHsp90+radicicol - yeast 1byq - hHsp90 NTD+ATP-Mg 1a4h – yHsp90+ geldamycin


Hsp82 is the yeast equivalent of Hsp90 in mammals

1ah6, 1ah8 – yHsp82 NTD 2wep – yHsp82 NTD (mutant)+ADP 2akp - yHsp82 NTD (mutant) 1weq, 3c11 - yHsp82 NTD (mutant)+ geldamycin 2wer - yHsp82 NTD (mutant)+radicicol 1bgq - yHsp82 NTD +radicicol 2iws, 2iwu, 2iwx, 2cgf - yHsp82 NTD +radicicol analogues 2vw5, 2vwc - yHsp82 NTD +7-o-carbamoylpremacbecin 3c0e - yHsp82 NTD (mutant) 2fxs - yHsp82 NTD +radamide 1zw9 - yHsp82 NTD + adenine derivative 1zwh - yHsp82 NTD + radester amine 2brc, 2bre - yHsp82 NTD+pyrazol derivative 2cg9 – yHsp82+co-chaperone SBA1 2cge - yHsp82 MD+CT+co-chaperone SBA1 1hk7 - yHsp82 MD 1usu, 1usv - yHsp82 MD+co-chaperone AHA1 1us7 - yHsp82 NTD+co-chaperone cdc37


Hsp100 are named ClpA or ClpB

1r6b, 1ksf – EcClpA (mutant) - Escherichia coli 1khy – EcClpB NTD 1jbk – EcClpB NBD 1k6k - EcClpA NTD


Hsp110 in mammals is named Sse1, Sse2 in yeast

3d2e, 3d2f, 3c7n – Sse1+Hsp70 – yeast 2qxl – Sse1 - yeast

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