1eb8
From Proteopedia
(New page: 200px<br /> <applet load="1eb8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eb8, resolution 2.10Å" /> '''STRUCTURE DETERMINA...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1EB8 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Manihot_esculenta Manihot esculenta]] with MPD as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.37 4.2.1.37]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EB8 OCA]]. | + | 1EB8 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Manihot_esculenta Manihot esculenta]] with MPD as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_3.3.2.4 Transferred entry: 3.3.2.4]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.37 4.2.1.37]]. Structure known Active Site: ASA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EB8 OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Manihot esculenta]] | [[Category: Manihot esculenta]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Transferred entry: 3.3.2.4]] | ||
[[Category: Effenberger, F.]] | [[Category: Effenberger, F.]] | ||
[[Category: Foerster, S.]] | [[Category: Foerster, S.]] | ||
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[[Category: substrate specificity]] | [[Category: substrate specificity]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:18:46 2007'' |
Revision as of 09:14, 30 October 2007
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STRUCTURE DETERMINANTS OF SUBSTRATE SPECIFICITY OF HYDROXYNITRILE LYASE FROM MANIHOT ESCULENTA
Overview
Tryptophan 128 of hydroxynitrile lyase of Manihot esculenta (MeHNL) covers, a significant part of a hydrophobic channel that gives access to the, active site of the enzyme. This residue was therefore substituted in the, mutant MeHNL-W128A by alanine to study its importance for the substrate, specificity of the enzyme. Wild-type MeHNL and MeHNL-W128A showed, comparable activity on the natural substrate acetone cyanohydrin (53 and, 40 U/mg, respectively). However, the specific activities of MeHNL-W128A, for the unnatural substrates mandelonitrile and 4-hydroxymandelonitrile, are increased 9-fold and approximately 450-fold, respectively, compared, with the wild-type MeHNL. The crystal structure of the MeHNL-W128A, substrate-free form at 2.1 A resolution indicates that the W128A, substitution ... [(full description)]
About this Structure
1EB8 is a [Single protein] structure of sequence from [Manihot esculenta] with MPD as [ligand]. Active as [Transferred entry: 3.3.2.4], with EC number [4.2.1.37]. Structure known Active Site: ASA. Full crystallographic information is available from [OCA].
Reference
Structure determinants of substrate specificity of hydroxynitrile lyase from Manihot esculenta., Lauble H, Miehlich B, Forster S, Kobler C, Wajant H, Effenberger F, Protein Sci. 2002 Jan;11(1):65-71. PMID:11742123
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