1ebe
From Proteopedia
(New page: 200px<br /> <applet load="1ebe" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ebe, resolution 2.2Å" /> '''LAUE DIFFRACTION STU...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1EBE is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ ]] with HEM and O as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.5 1.11.1.5]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EBE OCA]]. | + | 1EBE is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with HEM and O as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Cytochrome-c_peroxidase Cytochrome-c peroxidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.5 1.11.1.5]]. Structure known Active Sites: HEM and OXO. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EBE OCA]]. |
==Reference== | ==Reference== | ||
Laue diffraction study on the structure of cytochrome c peroxidase compound I., Fulop V, Phizackerley RP, Soltis SM, Clifton IJ, Wakatsuki S, Erman J, Hajdu J, Edwards SL, Structure. 1994 Mar 15;2(3):201-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8069633 8069633] | Laue diffraction study on the structure of cytochrome c peroxidase compound I., Fulop V, Phizackerley RP, Soltis SM, Clifton IJ, Wakatsuki S, Erman J, Hajdu J, Edwards SL, Structure. 1994 Mar 15;2(3):201-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8069633 8069633] | ||
| + | [[Category: Cytochrome-c peroxidase]] | ||
| + | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Clifton, I.J.]] | [[Category: Clifton, I.J.]] | ||
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[[Category: oxidoreductase (h2o2(a))]] | [[Category: oxidoreductase (h2o2(a))]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:18:51 2007'' |
Revision as of 09:14, 30 October 2007
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LAUE DIFFRACTION STUDY ON THE STRUCTURE OF CYTOCHROME C PEROXIDASE COMPOUND I
Overview
BACKGROUND: Cytochrome c peroxidase from yeast is a soluble, haem-containing protein found in the mitochondrial electron transport, chain where it probably protects against toxic peroxides. The aim of this, study was to obtain a reliable structure for the doubly oxidized transient, intermediate (termed compound I) in the reaction of cytochrome c, peroxidase with hydrogen peroxide. This intermediate contains a semistable, free radical on Trp191, and an oxyferryl haem group. RESULTS: Compound I, was produced in crystals of yeast cytochrome c peroxidase by reacting the, crystalline enzyme with hydrogen peroxide in a flow cell. The reaction was, monitored by microspectrophotometry and Laue crystallography in separate, experiments. A nearly complete conversion to compound I was achieved, ... [(full description)]
About this Structure
1EBE is a [Single protein] structure of sequence from [Saccharomyces cerevisiae] with HEM and O as [ligands]. Active as [Cytochrome-c peroxidase], with EC number [1.11.1.5]. Structure known Active Sites: HEM and OXO. Full crystallographic information is available from [OCA].
Reference
Laue diffraction study on the structure of cytochrome c peroxidase compound I., Fulop V, Phizackerley RP, Soltis SM, Clifton IJ, Wakatsuki S, Erman J, Hajdu J, Edwards SL, Structure. 1994 Mar 15;2(3):201-8. PMID:8069633
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