2qfa

From Proteopedia

Revision as of 10:23, 23 January 2008

Crystal structure of a Survivin-Borealin-INCENP core complex

Overview

The chromosomal passenger complex (CPC) is a key regulator of chromosome, segregation and cytokinesis. CPC functions are connected to its, localization. The complex first localizes to centromeres and later, associates with the central spindle and midbody. Survivin, Borealin, and, INCENP are the three components of the CPC that regulate the activity and, localization of its enzymatic component, the kinase Aurora B. We, determined the 1.4 A resolution crystal structure of the regulatory core, of the CPC, revealing that Borealin and INCENP associate with the helical, domain of Survivin to form a tight three-helical bundle. We used siRNA, rescue experiments with structure-based mutants to explore the, requirements for CPC localization. We show that the intertwined structural, interactions of the core components lead to functional interdependence., Association of the core "passenger" proteins creates a single structural, unit, whose composite molecular surface presents conserved residues, essential for central spindle and midbody localization.

About this Structure

2QFA is a Protein complex structure of sequences from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.

Reference

Structure of a Survivin-Borealin-INCENP Core Complex Reveals How Chromosomal Passengers Travel Together., Jeyaprakash AA, Klein UR, Lindner D, Ebert J, Nigg EA, Conti E, Cell. 2007 Oct 19;131(2):271-85. PMID:17956729

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