1mra
From Proteopedia
(New page: 200px<br /> <applet load="1mra" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mra, resolution 2.1Å" /> '''MANDELATE RACEMASE M...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1MRA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with MG and APG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.2 5.1.2.2]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MRA OCA]]. | + | 1MRA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with MG and APG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Mandelate_racemase Mandelate racemase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.2 5.1.2.2]]. Structure known Active Sites: ACT, CAR and MTL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MRA OCA]]. |
==Reference== | ==Reference== | ||
Mechanism of the reaction catalyzed by mandelate racemase: structure and mechanistic properties of the D270N mutant., Schafer SL, Barrett WC, Kallarakal AT, Mitra B, Kozarich JW, Gerlt JA, Clifton JG, Petsko GA, Kenyon GL, Biochemistry. 1996 May 7;35(18):5662-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8639525 8639525] | Mechanism of the reaction catalyzed by mandelate racemase: structure and mechanistic properties of the D270N mutant., Schafer SL, Barrett WC, Kallarakal AT, Mitra B, Kozarich JW, Gerlt JA, Clifton JG, Petsko GA, Kenyon GL, Biochemistry. 1996 May 7;35(18):5662-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8639525 8639525] | ||
| + | [[Category: Mandelate racemase]] | ||
[[Category: Pseudomonas putida]] | [[Category: Pseudomonas putida]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: mandelate pathway]] | [[Category: mandelate pathway]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:19:22 2007'' |
Revision as of 09:14, 30 October 2007
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MANDELATE RACEMASE MUTANT D270N CO-CRYSTALLIZED WITH (S)-ATROLACTATE
Overview
On the basis of the available high-resolution structures of mandelate, racemase (MR) from Pseudomonas putida [Landro, J.A., Gerlt, J.A., Kozarich, J.W., Koo, C.W., Shah, V.J., Kenyon, G.L., Neidhart, D.J., Fujita, J., & Petsko, G.A. (1994) Biochemistry 33, 635-643], Lys 166 and, His 297 are positioned appropriately to participate in catalysis as, acid/base catalysts, with Lys 166 participating as the (S)-specific, acid/base catalyst and His 297 participating as the (R)-specific acid/base, catalyst. The dependence of kcat on pH for the racemization of both (R)-, and (S)-mandelates suggests that the pKaS of the conjugate acids of Lys, 166 and His 297 are both approximately 6.4 [Landro, J.A., Kallarakal, A.T., Ransom, S.C., Gerlt, J.A., Kozarich, J.W., Neidhart, D.J., Kenyon, G.L. (1991) ... [(full description)]
About this Structure
1MRA is a [Single protein] structure of sequence from [Pseudomonas putida] with MG and APG as [ligands]. Active as [Mandelate racemase], with EC number [5.1.2.2]. Structure known Active Sites: ACT, CAR and MTL. Full crystallographic information is available from [OCA].
Reference
Mechanism of the reaction catalyzed by mandelate racemase: structure and mechanistic properties of the D270N mutant., Schafer SL, Barrett WC, Kallarakal AT, Mitra B, Kozarich JW, Gerlt JA, Clifton JG, Petsko GA, Kenyon GL, Biochemistry. 1996 May 7;35(18):5662-9. PMID:8639525
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