2q2u
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(New page: 200px<br /><applet load="2q2u" size="350" color="white" frame="true" align="right" spinBox="true" caption="2q2u, resolution 3.000Å" /> '''Structure of Chlore...)
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Revision as of 10:34, 23 January 2008
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Structure of Chlorella virus DNA ligase-product DNA complex
Overview
Chlorella virus DNA ligase, the smallest eukaryotic ligase known, has, pluripotent biological activity and an intrinsic nick-sensing function, despite having none of the accessory domains found in cellular ligases. A, 2.3-A crystal structure of the Chlorella virus ligase-AMP intermediate, bound to duplex DNA containing a 3'-OH-5'-PO4 nick reveals a new mode of, DNA envelopment, in which a short surface loop emanating from the OB, domain forms a beta-hairpin 'latch' that inserts into the DNA major groove, flanking the nick. A network of interactions with the 3'-OH and 5'-PO4, termini in the active site illuminates the DNA adenylylation mechanism and, the crucial roles of AMP in nick sensing and catalysis. Addition of a, divalent cation triggered nick sealing in crystallo, establishing that the, nick complex is a bona fide intermediate in the DNA repair pathway.
About this Structure
2Q2U is a Single protein structure of sequence from Paramecium bursaria chlorella virus 1. Full crystallographic information is available from OCA.
Reference
Structural basis for nick recognition by a minimal pluripotent DNA ligase., Nair PA, Nandakumar J, Smith P, Odell M, Lima CD, Shuman S, Nat Struct Mol Biol. 2007 Aug;14(8):770-8. Epub 2007 Jul 8. PMID:17618295
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