2jgp
From Proteopedia
(New page: 200px<br /><applet load="2jgp" size="450" color="white" frame="true" align="right" spinBox="true" caption="2jgp, resolution 1.85Å" /> '''STRUCTURE OF THE TYC...) |
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| - | [[Image:2jgp.gif|left|200px]]<br /><applet load="2jgp" size=" | + | [[Image:2jgp.gif|left|200px]]<br /><applet load="2jgp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2jgp, resolution 1.85Å" /> | caption="2jgp, resolution 1.85Å" /> | ||
'''STRUCTURE OF THE TYCC5-6 PCP-C BIDOMAIN OF THE TYROCIDINE SYNTHETASE TYCC'''<br /> | '''STRUCTURE OF THE TYCC5-6 PCP-C BIDOMAIN OF THE TYROCIDINE SYNTHETASE TYCC'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2JGP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Brevibacillus_brevis Brevibacillus brevis] with SO4, NA and DIO as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:So4 Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:So4 Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:Dio Binding Site For Chain A'>AC3</scene>, <scene name='pdbsite=AC4:Dio Binding Site For Chain A'>AC4</scene> and <scene name='pdbsite=AC5:Na Binding Site For Chain A'>AC5</scene>. Full crystallographic information is available from [http:// | + | 2JGP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Brevibacillus_brevis Brevibacillus brevis] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=DIO:'>DIO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:So4 Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:So4 Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:Dio Binding Site For Chain A'>AC3</scene>, <scene name='pdbsite=AC4:Dio Binding Site For Chain A'>AC4</scene> and <scene name='pdbsite=AC5:Na Binding Site For Chain A'>AC5</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JGP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: tyrocidine]] | [[Category: tyrocidine]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:35:51 2008'' |
Revision as of 10:35, 23 January 2008
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STRUCTURE OF THE TYCC5-6 PCP-C BIDOMAIN OF THE TYROCIDINE SYNTHETASE TYCC
Overview
The crystal structure of the bidomain PCP-C from modules 5 and 6 of the, nonribosomal tyrocidine synthetase TycC was determined at 1.8 A, resolution. The bidomain structure reveals a V-shaped condensation domain, the canyon-like active site groove of which is associated with the, preceding peptidyl carrier protein (PCP) domain at its donor side. The, relative arrangement of the PCP and the peptide bond-forming condensation, (C) domain places the active sites approximately 50 A apart. Accordingly, this PCP-C structure represents a conformational state prior to peptide, transfer from the donor-PCP to the acceptor-PCP domain, implying the, existence of additional states of PCP-C domain interaction during, catalysis. Additionally, PCP-C exerts a mode of cyclization activity that, mimics peptide bond formation catalyzed by C domains. Based on mutational, data and pK value analysis of active site residues, it is suggested that, nonribosomal peptide bond formation depends on electrostatic interactions, rather than on general acid/base catalysis.
About this Structure
2JGP is a Single protein structure of sequence from Brevibacillus brevis with , and as ligands. Known structural/functional Sites: , , , and . Full crystallographic information is available from OCA.
Reference
Structural and functional insights into a peptide bond-forming bidomain from a nonribosomal peptide synthetase., Samel SA, Schoenafinger G, Knappe TA, Marahiel MA, Essen LO, Structure. 2007 Jul;15(7):781-92. PMID:17637339
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Categories: Brevibacillus brevis | Single protein | Essen, L.O. | Knappe, T.A. | Marahiel, M.A. | Samel, S.A. | Schoenafinger, G. | DIO | NA | SO4 | Antibiotic biosynthesis | Antibiotics | Condensation domain | Ligase | Multifunctional enzyme | Nonribosomal peptide synthetase | Peptide bond formation | Peptidyl carrier domain | Phosphopantetheine | Tyrocidine
