2pjd
From Proteopedia
(New page: 200px<br /><applet load="2pjd" size="450" color="white" frame="true" align="right" spinBox="true" caption="2pjd, resolution 2.10Å" /> '''Crystal structure of...) |
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caption="2pjd, resolution 2.10Å" /> | caption="2pjd, resolution 2.10Å" /> | ||
'''Crystal structure of 16S rRNA methyltransferase RsmC'''<br /> | '''Crystal structure of 16S rRNA methyltransferase RsmC'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PJD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/rRNA_(guanine-N(2)-)-methyltransferase rRNA (guanine-N(2)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.52 2.1.1.52] Full crystallographic information is available from [http:// | + | 2PJD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/rRNA_(guanine-N(2)-)-methyltransferase rRNA (guanine-N(2)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.52 2.1.1.52] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PJD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
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Revision as of 10:41, 23 January 2008
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Crystal structure of 16S rRNA methyltransferase RsmC
Overview
RNA methyltransferases (MTases) are important players in the biogenesis, and regulation of the ribosome, the cellular machine for protein, synthesis. RsmC is a MTase that catalyzes the transfer of a methyl group, from S-adenosyl-l-methionine (SAM) to G1207 of 16S rRNA. Mutations of, G1207 have dominant lethal phenotypes in Escherichia coli, underscoring, the significance of this modified nucleotide for ribosome function. Here, we report the crystal structure of E. coli RsmC refined to 2.1 A, resolution, which reveals two homologous domains tandemly duplicated, within a single polypeptide. We characterized the function of the, individual domains and identified key residues involved in binding of rRNA, and SAM, and in catalysis. We also discovered that one of the domains is, important for the folding of the other. Domain duplication and, subfunctionalization by complementary degeneration of redundant functions, (in particular substrate binding versus catalysis) has been reported for, many enzymes, including those involved in RNA metabolism. Thus, RsmC can, be regarded as a model system for functional streamlining of domains, accompanied by the development of dependencies concerning folding and, stability.
About this Structure
2PJD is a Single protein structure of sequence from Escherichia coli. Active as rRNA (guanine-N(2)-)-methyltransferase, with EC number 2.1.1.52 Full crystallographic information is available from OCA.
Reference
Functional specialization of domains tandemly duplicated within 16S rRNA methyltransferase RsmC., Sunita S, Purta E, Durawa M, Tkaczuk KL, Swaathi J, Bujnicki JM, Sivaraman J, Nucleic Acids Res. 2007 Jun 27;. PMID:17576679
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