2qre

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Revision as of 10:44, 23 January 2008

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spinqualitylabelsall models 2qre, resolution 3.01Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide (ZMP)

Overview

The AMP-activated protein kinase (AMPK), a sensor of cellular energy, status found in all eukaryotes, responds to changes in intracellular, adenosine nucleotide levels resulting from metabolic stresses. Here we, describe crystal structures of a heterotrimeric regulatory core fragment, from Schizosaccharomyces pombe AMPK in complex with ADP, ADP/AMP, ADP/ATP, and 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide (AICAR, phosphate, or ZMP), a well-characterized AMPK activator. Prior, crystallographic studies had revealed a single site in the gamma subunit, that binds either ATP or AMP within Bateman domain B. Here we show that, ZMP binds at this site, mimicking the binding of AMP. An analogous site in, Bateman domain A selectively accommodates ADP, which binds in a distinct, manner that also involves direct ligation to elements from the beta, subunit. These observations suggest a possible role for ADP in regulating, AMPK response to changes in cellular energy status.

About this Structure

2QRE is a Protein complex structure of sequences from Schizosaccharomyces pombe with as ligand. Active as Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 Full crystallographic information is available from OCA.

Reference

Structural Insight into AMPK Regulation: ADP Comes into Play., Jin X, Townley R, Shapiro L, Structure. 2007 Oct;15(10):1285-95. PMID:17937917

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