2cju
From Proteopedia
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| - | [[Image:2cju.gif|left|200px]]<br /><applet load="2cju" size=" | + | [[Image:2cju.gif|left|200px]]<br /><applet load="2cju" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2cju, resolution 2.5Å" /> | caption="2cju, resolution 2.5Å" /> | ||
'''CRYSTAL STRUCTURE OF THE TEPC15-VK45.1 ANTI-2-PHENYL-5-OXAZOLONE NQ16-113.8 SCFV IN COMPLEX WITH PHOXGABA'''<br /> | '''CRYSTAL STRUCTURE OF THE TEPC15-VK45.1 ANTI-2-PHENYL-5-OXAZOLONE NQ16-113.8 SCFV IN COMPLEX WITH PHOXGABA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2CJU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with PHX as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Phx Binding Site For Chain H'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2CJU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=PHX:'>PHX</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Phx Binding Site For Chain H'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: scfv]] | [[Category: scfv]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:47:54 2008'' |
Revision as of 10:47, 23 January 2008
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CRYSTAL STRUCTURE OF THE TEPC15-VK45.1 ANTI-2-PHENYL-5-OXAZOLONE NQ16-113.8 SCFV IN COMPLEX WITH PHOXGABA
Overview
Affinity maturation is a process that leads to the emergence of more, efficient antibodies following initial antigen encounter and represents a, key strategy of the adaptive immunity of vertebrate organisms. Earlier and, detailed sequence studies of the antibody response to a model antigen, the, hapten 2-phenyl-5-oxazolone (phOx), define three different classes of, antibodies. Class I antibodies use the V(H)Ox1/V(kappa)Ox1 gene pair and, dominate the early stages of the anti-phOx response, class II antibodies, use the V(kappa)Ox1 gene but a different V(H) segment and are common in, the intermediate stages, and class III antibodies use the, TEPC15/V(kappa)45.1 genes and play the greatest role in the late stages., Only the crystal structure of one anti-phOx antibody, the class II, NQ10/12.5 Fab fragment, has been described. Here we report the crystal, structures of the scFv form of the low and high affinity anti-phOx class, III antibodies NQ10/1.12 and NQ16/113.8 complexed with the hapten. The two, antibodies differ by nine amino acid substitutions, all located in the, V(H) domain. Analysis of the two structures shows that affinity maturation, results from an increase in surface complementarity, as a consequence of a, finely tuned and highly concerted process chaperoned by the somatic, mutations, and implies a more efficient hapten-induced fit in the mature, antibody. The data also demonstrate that class III antibodies respond in a, completely different way to the architectural problem of binding phOx, compared to the class II antibody NQ10/12.5.
About this Structure
2CJU is a Single protein structure of sequence from Mus musculus with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural basis of affinity maturation of the TEPC15/Vkappa45.1 anti-2-phenyl-5-oxazolone antibodies., Scotti C, Gherardi E, J Mol Biol. 2006 Jun 23;359(5):1161-9. Epub 2006 May 2. PMID:16682055
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