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2hu9
From Proteopedia
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(New page: 200px<br /><applet load="2hu9" size="350" color="white" frame="true" align="right" spinBox="true" caption="2hu9, resolution 1.780Å" /> '''X-ray structure of ...)
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Revision as of 10:48, 23 January 2008
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X-ray structure of the Archaeoglobus fulgidus CopZ N-terminal Domain
Overview
Bacterial CopZ proteins deliver copper to P1B-type Cu+-ATPases that are, homologous to the human Wilson and Menkes disease proteins. The genome of, the hyperthermophile Archaeoglobus fulgidus encodes a putative CopZ copper, chaperone that contains an unusual cysteine-rich N-terminal domain of 130, amino acids in addition to a C-terminal copper binding domain with a, conserved CXXC motif. The N-terminal domain (CopZ-NT) is homologous to, proteins found only in extremophiles and is the only such protein that is, fused to a copper chaperone. Surprisingly, optical, electron paramagnetic, resonance, and x-ray absorption spectroscopic data indicate the presence, of a [2Fe-2S] cluster in CopZ-NT. The intact CopZ protein binds two copper, ions, one in each domain. The 1.8 A resolution crystal structure of, CopZ-NT reveals that the [2Fe-2S] cluster is housed within a novel fold, and that the protein also binds a zinc ion at a four-cysteine site. CopZ, can deliver Cu+ to the A. fulgidus CopA N-terminal metal binding domain, and is capable of reducing Cu2+ to Cu+. This unique fusion of a, redox-active domain with a CXXC-containing copper chaperone domain is, relevant to the evolution of copper homeostatic mechanisms and suggests, new models for copper trafficking.
About this Structure
2HU9 is a Single protein structure of sequence from Archaeoglobus fulgidus with , and as ligands. Full crystallographic information is available from OCA.
Reference
Characterization and structure of a Zn2+ and [2Fe-2S]-containing copper chaperone from Archaeoglobus fulgidus., Sazinsky MH, LeMoine B, Orofino M, Davydov R, Bencze KZ, Stemmler TL, Hoffman BM, Arguello JM, Rosenzweig AC, J Biol Chem. 2007 Aug 31;282(35):25950-9. Epub 2007 Jul 3. PMID:17609202
Page seeded by OCA on Wed Jan 23 12:48:54 2008
Categories: Archaeoglobus fulgidus | Single protein | Arguello, J.M. | LeMoine, B. | Rosenzweig, A.C. | Sazinsky, M.H. | ACY | FES | ZN | Atox1 | Atx1 | Copper chaperone | Copz | Iron-sufur protein | Metal transport
