2p73
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(New page: 200px<br /><applet load="2p73" size="350" color="white" frame="true" align="right" spinBox="true" caption="2p73, resolution 2.300Å" /> '''crystal structure o...)
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Revision as of 10:50, 23 January 2008
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crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1
Overview
Paramecium bursaria chlorella virus-1 encodes at least five putative, glycosyltransferases that are probably involved in the synthesis of the, glycan components of the viral major capsid protein. The 1.6 A crystal, structure of one of these glycosyltransferases (A64R) has a mixed, alpha/beta fold containing a central, six-stranded beta sheet flanked by, alpha helices. Crystal structures of A64R, complexed with UDP, CMP, or, GDP, established that only UDP bound to A64R in the presence of Mn(2+), consistent with its high structural similarity to glycosyltransferases, which utilize UDP as the sugar carrier. The structure of the complex of, A64R, UDP-glucose, and Mn(2+) showed that the largest conformational, change occurred when hydrogen bonds were formed with the ligands. Unlike, UDP-glucose, UDP-galactose and UDP-GlcNAc did not bind to A64R, suggesting, a selective binding of UDP-glucose. Thus, UDP-glucose is most likely the, sugar donor for A64R, consistent with glucose occurring in the virus major, capsid protein glycans.
About this Structure
2P73 is a Single protein structure of sequence from Paramecium bursaria chlorella virus 1 with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure and function of a chlorella virus-encoded glycosyltransferase., Zhang Y, Xiang Y, Van Etten JL, Rossmann MG, Structure. 2007 Sep;15(9):1031-9. PMID:17850743
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