2z9q
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(New page: 200px<br /><applet load="2z9q" size="350" color="white" frame="true" align="right" spinBox="true" caption="2z9q" /> '''Transfer RNA in the hybrid P/E state'''<br /...)
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Revision as of 10:55, 23 January 2008
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Transfer RNA in the hybrid P/E state
Overview
During the ribosomal translocation, the binding of elongation factor G, (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation, of the 30S subunit relative to the 50S subunit in the direction of the, mRNA movement. By means of cryo-electron microscopy we observe that this, rotation is accompanied by a 20 A movement of the L1 stalk of the 50S, subunit, implying that this region is involved in the translocation of, deacylated tRNAs from the P to the E site. These ribosomal motions can, occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer, to the A-site tRNA or peptide removal, the presence of the charged P-site, tRNA locks the ribosome and prohibits both of these motions.
About this Structure
2Z9Q is a Single protein structure of sequence from Thermus aquaticus. Full crystallographic information is available from OCA.
Reference
Locking and unlocking of ribosomal motions., Valle M, Zavialov A, Sengupta J, Rawat U, Ehrenberg M, Frank J, Cell. 2003 Jul 11;114(1):123-34. PMID:12859903
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