2v1u
From Proteopedia
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| - | [[Image:2v1u.gif|left|200px]]<br /><applet load="2v1u" size=" | + | [[Image:2v1u.gif|left|200px]]<br /><applet load="2v1u" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2v1u, resolution 3.10Å" /> | caption="2v1u, resolution 3.10Å" /> | ||
'''STRUCTURE OF THE AEROPYRUM PERNIX ORC1 PROTEIN IN COMPLEX WITH DNA'''<br /> | '''STRUCTURE OF THE AEROPYRUM PERNIX ORC1 PROTEIN IN COMPLEX WITH DNA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2V1U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Aeropyrum_pernix Aeropyrum pernix] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2V1U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Aeropyrum_pernix Aeropyrum pernix] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V1U OCA]. |
==Reference== | ==Reference== | ||
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[[Category: replication]] | [[Category: replication]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:58:46 2008'' |
Revision as of 10:58, 23 January 2008
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STRUCTURE OF THE AEROPYRUM PERNIX ORC1 PROTEIN IN COMPLEX WITH DNA
Overview
DNA replication in archaea and in eukaryotes share many similarities. We, report the structure of an archaeal origin recognition complex protein, ORC1, bound to an origin recognition box, a DNA sequence that is found in, multiple copies at replication origins. DNA binding is mediated, principally by a C-terminal winged helix domain that inserts deeply into, the major and minor grooves, widening them both. However, additional DNA, contacts are made with the N-terminal AAA+ domain, which inserts into the, minor groove at a characteristic G-rich sequence, inducing a 35 degrees, bend in the duplex and providing directionality to the binding site. Both, contact regions also induce substantial unwinding of the DNA. The, structure provides insight into the initial step in assembly of a, replication origin and recruitment of minichromosome maintenance (MCM), helicase to that origin.
About this Structure
2V1U is a Protein complex structure of sequences from Aeropyrum pernix with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural basis of DNA replication origin recognition by an ORC protein., Gaudier M, Schuwirth BS, Westcott SL, Wigley DB, Science. 2007 Aug 31;317(5842):1213-6. PMID:17761880
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