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2j18
From Proteopedia
(New page: 200px<br /> <applet load="2j18" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j18, resolution 1.75Å" /> '''CHLOROPEROXIDASE MI...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2J18 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Leptoxyphium_fumago Leptoxyphium fumago]] with NAG, MAN, MN, BR and HEM as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J18 OCA]]. | + | 2J18 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Leptoxyphium_fumago Leptoxyphium fumago]] with NAG, MAN, MN, BR and HEM as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J18 OCA]]. |
==Reference== | ==Reference== | ||
Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography., Beitlich T, Kuhnel K, Schulze-Briese C, Shoeman RL, Schlichting I, J Synchrotron Radiat. 2007 Jan;14(Pt 1):11-23. Epub 2006 Dec 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17211068 17211068] | Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography., Beitlich T, Kuhnel K, Schulze-Briese C, Shoeman RL, Schlichting I, J Synchrotron Radiat. 2007 Jan;14(Pt 1):11-23. Epub 2006 Dec 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17211068 17211068] | ||
| + | [[Category: Chloride peroxidase]] | ||
[[Category: Leptoxyphium fumago]] | [[Category: Leptoxyphium fumago]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: pyrrolidone carboxylic acid]] | [[Category: pyrrolidone carboxylic acid]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:26:44 2007'' |
Revision as of 09:22, 30 October 2007
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CHLOROPEROXIDASE MIXTURE OF FERRIC AND FERROUS STATES (LOW DOSE DATA SET)
Overview
The X-ray crystallographic analysis of redox-active systems may be, complicated by photoreduction. Although radiolytic reduction by the, probing X-ray beam may be exploited to generate otherwise short-lived, reaction intermediates of metalloproteins, it is generally an undesired, feature. Here, the X-ray-induced reduction of the three heme proteins, myoglobin, cytochrome P450cam and chloroperoxidase has been followed by, on-line UV-Vis absorption spectroscopy. All three systems showed a very, rapid reduction of the heme iron. In chloroperoxidase the change of the, ionization state from ferric to ferrous heme is associated with a movement, of the heme-coordinating water molecule. The influence of the energy of, the incident X-ray photons and of the presence of scavengers on the, apparent ... [(full description)]
About this Structure
2J18 is a [Single protein] structure of sequence from [Leptoxyphium fumago] with NAG, MAN, MN, BR and HEM as [ligands]. Active as [Chloride peroxidase], with EC number [1.11.1.10]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography., Beitlich T, Kuhnel K, Schulze-Briese C, Shoeman RL, Schlichting I, J Synchrotron Radiat. 2007 Jan;14(Pt 1):11-23. Epub 2006 Dec 15. PMID:17211068
Page seeded by OCA on Tue Oct 30 11:26:44 2007
Categories: Chloride peroxidase | Leptoxyphium fumago | Single protein | Beitlich, T. | Kuhnel, K. | Schlichting, I. | Schulze-Briese, C. | Shoeman, R.L. | BR | HEM | MAN | MN | NAG | Chloride | Glycoprotein | Heme | Iron | Manganese | Metal-binding | Oxidoreductase | Peroxidase | Pyrrolidone carboxylic acid
