2d5i
From Proteopedia
(New page: 200px<br /><applet load="2d5i" size="450" color="white" frame="true" align="right" spinBox="true" caption="2d5i, resolution 2.20Å" /> '''The crystal structur...) |
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| - | [[Image:2d5i.gif|left|200px]]<br /><applet load="2d5i" size=" | + | [[Image:2d5i.gif|left|200px]]<br /><applet load="2d5i" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2d5i, resolution 2.20Å" /> | caption="2d5i, resolution 2.20Å" /> | ||
'''The crystal structure of AzoR (Azo Reductase) from Escherichia coli'''<br /> | '''The crystal structure of AzoR (Azo Reductase) from Escherichia coli'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2D5I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with FMN and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Azobenzene_reductase Azobenzene reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.1.6 1.7.1.6] Full crystallographic information is available from [http:// | + | 2D5I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=FMN:'>FMN</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Azobenzene_reductase Azobenzene reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.1.6 1.7.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D5I OCA]. |
==Reference== | ==Reference== | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:06:17 2008'' |
Revision as of 11:06, 23 January 2008
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The crystal structure of AzoR (Azo Reductase) from Escherichia coli
Overview
The crystal structure of AzoR (azoreductase) has been determined in, complex with FMN for two different crystal forms at 1.8 and 2.2 A, resolution. AzoR is an oxidoreductase isolated from Escherichia coli as a, protein responsible for the degradation of azo compounds. This enzyme is, an FMN-dependent NADH-azoreductase and catalyzes the reductive cleavage of, azo groups by a ping-pong mechanism. The structure suggests that AzoR acts, in a homodimeric state forming the two identical catalytic sites to which, both monomers contribute. The structure revealed that each monomer of AzoR, has a flavodoxin-like structure, without the explicit overall amino acid, sequence homology. Superposition of the structures from the two different, crystal forms revealed the conformational change and suggested a mechanism, for accommodating substrates of different size. Furthermore, comparison of, the active site structure with that of NQO1 complexed with substrates, provides clues to the possible substrate-binding mechanism of AzoR.
About this Structure
2D5I is a Single protein structure of sequence from Escherichia coli with and as ligands. Active as Azobenzene reductase, with EC number 1.7.1.6 Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of AzoR from Escherichia coli. An oxidereductase conserved in microorganisms., Ito K, Nakanishi M, Lee WC, Sasaki H, Zenno S, Saigo K, Kitade Y, Tanokura M, J Biol Chem. 2006 Jul 21;281(29):20567-76. Epub 2006 May 9. PMID:16684776
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