2e2x
From Proteopedia
(New page: 200px<br /> <applet load="2e2x" size="450" color="white" frame="true" align="right" spinBox="true" caption="2e2x, resolution 2.5Å" /> '''Sec14 Homology Modul...) |
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caption="2e2x, resolution 2.5Å" /> | caption="2e2x, resolution 2.5Å" /> | ||
'''Sec14 Homology Module of Neurofibromin in complex with phosphatitylethanolamine'''<br /> | '''Sec14 Homology Module of Neurofibromin in complex with phosphatitylethanolamine'''<br /> | ||
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==Overview== | ==Overview== | ||
Neurofibromin is the protein product of the tumor suppressor gene NF1, alterations of which are responsible for the pathogenesis of the common, disorder Neurofibromatosis type I (NF1). The only well-characterized, function of neurofibromin is its RasGAP activity, contained in the central, GAP related domain (GRD). By solving the crystal structure of a 31 kDa, fragment at the C-terminal end of the GRD we have recently identified a, novel bipartite lipid-binding module composed of a Sec14 homologous and a, previously undetected pleckstrin homology (PH)-like domain. Using lipid, exchange assays along with mass spectrometry we show here that the, Sec14-like portion binds to 1-(3-sn-phosphatidyl)-sn-glycerol (PtdGro), (3-sn-phosphatidyl)-ethanolamine (PtdEtn) and -choline (PtdCho) and to a, minor extent to (3-sn-phosphatidyl)-l-serine (PtdSer) and, 1-(3-sn-phosphatidyl)-d-myo-inositol (PtdIns). Phosphorylated PtdIns, (PtdInsPs) are not detected as binders in the mass spectrometry assay, but, their soluble inositol-phosphate headgroups and related compounds can, inhibit the lipid exchange reaction. We also present here the crystal, structure of this module with the Sec14 portion bound to a cellular, glycerophospholipid ligand. Our structure has model character for the, substrate-bound form of yeast Sec14p, of which only detergent bound, structures are available so far. To assess potential regulation of the, lipid exchange reaction in detail, we present a novel strategy using, nanospray mass spectrometry. Ion intensities of initial phospholipids and, exchanged deuterated analogues bound by the protein module allow the, quantitative analysis of differences in the exchange activity under, various conditions. | Neurofibromin is the protein product of the tumor suppressor gene NF1, alterations of which are responsible for the pathogenesis of the common, disorder Neurofibromatosis type I (NF1). The only well-characterized, function of neurofibromin is its RasGAP activity, contained in the central, GAP related domain (GRD). By solving the crystal structure of a 31 kDa, fragment at the C-terminal end of the GRD we have recently identified a, novel bipartite lipid-binding module composed of a Sec14 homologous and a, previously undetected pleckstrin homology (PH)-like domain. Using lipid, exchange assays along with mass spectrometry we show here that the, Sec14-like portion binds to 1-(3-sn-phosphatidyl)-sn-glycerol (PtdGro), (3-sn-phosphatidyl)-ethanolamine (PtdEtn) and -choline (PtdCho) and to a, minor extent to (3-sn-phosphatidyl)-l-serine (PtdSer) and, 1-(3-sn-phosphatidyl)-d-myo-inositol (PtdIns). Phosphorylated PtdIns, (PtdInsPs) are not detected as binders in the mass spectrometry assay, but, their soluble inositol-phosphate headgroups and related compounds can, inhibit the lipid exchange reaction. We also present here the crystal, structure of this module with the Sec14 portion bound to a cellular, glycerophospholipid ligand. Our structure has model character for the, substrate-bound form of yeast Sec14p, of which only detergent bound, structures are available so far. To assess potential regulation of the, lipid exchange reaction in detail, we present a novel strategy using, nanospray mass spectrometry. Ion intensities of initial phospholipids and, exchanged deuterated analogues bound by the protein module allow the, quantitative analysis of differences in the exchange activity under, various conditions. | ||
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| - | ==Disease== | ||
| - | Known diseases associated with this structure: Leukemia, juvenile myelomonocytic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=162200 162200]], Melanoma, desmoplastic neurotropic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=162200 162200]], Neurofibromatosis, familial spinal OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=162200 162200]], Neurofibromatosis, type 1 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=162200 162200]], Neurofibromatosis-Noonan syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=162200 162200]], Pseudarthrosis, tibial, in NF1 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=162200 162200]], Watson syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=162200 162200]] | ||
==About this Structure== | ==About this Structure== | ||
| - | 2E2X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with PEV and POP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2E2X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=PEV:'>PEV</scene> and <scene name='pdbligand=POP:'>POP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E2X OCA]. |
==Reference== | ==Reference== | ||
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[[Category: signaling protein]] | [[Category: signaling protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:09:02 2008'' |
Revision as of 11:09, 23 January 2008
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Sec14 Homology Module of Neurofibromin in complex with phosphatitylethanolamine
Overview
Neurofibromin is the protein product of the tumor suppressor gene NF1, alterations of which are responsible for the pathogenesis of the common, disorder Neurofibromatosis type I (NF1). The only well-characterized, function of neurofibromin is its RasGAP activity, contained in the central, GAP related domain (GRD). By solving the crystal structure of a 31 kDa, fragment at the C-terminal end of the GRD we have recently identified a, novel bipartite lipid-binding module composed of a Sec14 homologous and a, previously undetected pleckstrin homology (PH)-like domain. Using lipid, exchange assays along with mass spectrometry we show here that the, Sec14-like portion binds to 1-(3-sn-phosphatidyl)-sn-glycerol (PtdGro), (3-sn-phosphatidyl)-ethanolamine (PtdEtn) and -choline (PtdCho) and to a, minor extent to (3-sn-phosphatidyl)-l-serine (PtdSer) and, 1-(3-sn-phosphatidyl)-d-myo-inositol (PtdIns). Phosphorylated PtdIns, (PtdInsPs) are not detected as binders in the mass spectrometry assay, but, their soluble inositol-phosphate headgroups and related compounds can, inhibit the lipid exchange reaction. We also present here the crystal, structure of this module with the Sec14 portion bound to a cellular, glycerophospholipid ligand. Our structure has model character for the, substrate-bound form of yeast Sec14p, of which only detergent bound, structures are available so far. To assess potential regulation of the, lipid exchange reaction in detail, we present a novel strategy using, nanospray mass spectrometry. Ion intensities of initial phospholipids and, exchanged deuterated analogues bound by the protein module allow the, quantitative analysis of differences in the exchange activity under, various conditions.
About this Structure
2E2X is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
The sec14 homology module of neurofibromin binds cellular glycerophospholipids: mass spectrometry and structure of a lipid complex., Welti S, Fraterman S, D'Angelo I, Wilm M, Scheffzek K, J Mol Biol. 2007 Feb 16;366(2):551-62. Epub 2006 Nov 18. PMID:17187824
Page seeded by OCA on Wed Jan 23 13:09:02 2008
Categories: Homo sapiens | Single protein | Angelo, I.D. | Scheffzek, K. | Welti, S. | PEV | POP | Cral-trio domain | Pe | Ph superfold | Sec14 | Signaling protein
