This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2ip6
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="2ip6" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ip6, resolution 1.35Å" /> '''Crystal structure of...)
Next diff →
Revision as of 11:17, 23 January 2008
|
Crystal structure of PedB
Overview
BACKGROUND: Pediocin-like bacteriocins, ribosomally-synthesized, antimicrobial peptides, are generally coexpressed with cognate immunity, proteins in order to protect the bacteriocin-producer from its own, bacteriocin. As a step for understanding the mode of action of immunity, proteins, we determined the crystal structure of PedB, a pediocin-like, immunity protein conferring immunity to pediocin PP-1. RESULTS: The 1.6 A, crystal structure of PedB reveals that PedB consists of an antiparallel, four-helix bundle with a flexible C-terminal end. PedB shows structural, similarity to an immunity protein against enterocin A (EntA-im) but some, disparity to an immunity protein against carnobacteriocin B2 (ImB2) in, both the C-terminal conformation and the local structure constructed by, alpha3, alpha4, and their connecting loop. Structure-inspired mutational, studies reveal that deletion of the last seven residues of the C-terminus, of PedB almost abolished its immunity activity. CONCLUSION: The fact that, PedB, EntA-im, and ImB2 share a four-helix bundle structure strongly, suggests the structural conservation of this motif in the pediocin-like, immunity proteins. The significant difference in the core structure and, the C-terminal conformation provides a structural basis for the, classification of pediocin-like immunity proteins. Our mutational study, using C-terminal-shortened PedBs and the investigation of primary sequence, of the C-terminal region, propose that several polar or charged residues, in the extreme C-terminus of PedB which is crucial for the immunity are, involved in the specific recognition of pediocin PP-1.
About this Structure
2IP6 is a Single protein structure of sequence from Pediococcus pentosaceus with as ligand. Full crystallographic information is available from OCA.
Reference
High resolution crystal structure of PedB: a structural basis for the classification of pediocin-like immunity proteins., Kim IK, Kim MK, Kim JH, Yim HS, Cha SS, Kang SO, BMC Struct Biol. 2007 May 30;7:35. PMID:17537233
Page seeded by OCA on Wed Jan 23 13:17:32 2008
