2qjz
From Proteopedia
(New page: 200px<br /> <applet load="2qjz" size="450" color="white" frame="true" align="right" spinBox="true" caption="2qjz, resolution 1.250Å" /> '''Structural Basis o...) |
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| - | [[Image:2qjz.gif|left|200px]]<br /> | + | [[Image:2qjz.gif|left|200px]]<br /><applet load="2qjz" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="2qjz, resolution 1.250Å" /> | caption="2qjz, resolution 1.250Å" /> | ||
'''Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1'''<br /> | '''Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2QJZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 2QJZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QJZ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein binding]] | [[Category: protein binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:21:21 2008'' |
Revision as of 11:21, 23 January 2008
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Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1
Overview
Microtubule plus end binding proteins (+TIPs) localize to the dynamic plus, ends of microtubules, where they stimulate microtubule growth and recruit, signaling molecules. Three main +TIP classes have been identified, (XMAP215, EB1, and CLIP-170), but whether they act upon microtubule plus, ends through a similar mechanism has not been resolved. Here, we report, crystal structures of the tubulin binding domains of XMAP215 (yeast Stu2p, and Drosophila Msps), EB1 (yeast Bim1p and human EB1), and CLIP-170, (human), which reveal diverse tubulin binding interfaces. Functional, studies, however, reveal a common property that native or artificial, dimerization of tubulin binding domains (including chemically induced, heterodimers of EB1 and CLIP-170) induces tubulin nucleation/assembly in, vitro and, in most cases, plus end tracking in living cells. We propose, that +TIPs, although diverse in structure, share a common property of, multimerizing tubulin, thus acting as polymerization chaperones that aid, in subunit addition to the microtubule plus end.
About this Structure
2QJZ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170, and EB1., Slep KC, Vale RD, Mol Cell. 2007 Sep 21;27(6):976-91. PMID:17889670
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