2vaz

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Revision as of 11:23, 23 January 2008

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spinqualitylabelsall models 2vaz, resolution 10.0Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

MODEL OF THE S15-MRNA COMPLEX FITTED INTO THE CRYO-EM MAP OF THE 70S ENTRAPMENT COMPLEX.

Overview

Gene expression can be regulated at the level of initiation of protein, biosynthesis via structural elements present at the 5' untranslated region, of mRNAs. These folded mRNA segments may bind to the ribosome, thus, blocking translation until the mRNA unfolds. Here, we report a series of, cryo-electron microscopy snapshots of ribosomal complexes directly, visualizing either the mRNA structure blocked by repressor protein S15 or, the unfolded, active mRNA. In the stalled state, the folded mRNA prevents, the start codon from reaching the peptidyl-tRNA (P) site inside the, ribosome. Upon repressor release, the mRNA unfolds and moves into the mRNA, channel allowing translation initiation. A comparative structure and, sequence analysis suggests the existence of a universal stand-by site on, the ribosome (the 30S platform) dedicated for binding regulatory 5' mRNA, elements. Different types of mRNA structures may be accommodated during, translation preinitiation and regulate gene expression by transiently, stalling the ribosome.

About this Structure

2VAZ is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structured mRNAs Regulate Translation Initiation by Binding to the Platform of the Ribosome., Marzi S, Myasnikov AG, Serganov A, Ehresmann C, Romby P, Yusupov M, Klaholz BP, Cell. 2007 Sep 21;130(6):1019-1031. PMID:17889647

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