1dt7
From Proteopedia
(New page: 200px<br /> <applet load="1dt7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dt7" /> '''SOLUTION STRUCTURE OF THE C-TERMINAL NEGATI...) |
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'''SOLUTION STRUCTURE OF THE C-TERMINAL NEGATIVE REGULATORY DOMAIN OF P53 IN A COMPLEX WITH CA2+-BOUND S100B(BB)'''<br /> | '''SOLUTION STRUCTURE OF THE C-TERMINAL NEGATIVE REGULATORY DOMAIN OF P53 IN A COMPLEX WITH CA2+-BOUND S100B(BB)'''<br /> | ||
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==Overview== | ==Overview== | ||
A Ca2+ dependent conformational change in dimeric S100B(betabeta) is, required for it to bind p53 and inhibit phosphorylation of this tumor, suppressor in its C-terminal negative regulatory domain. A peptide derived, from this region of p53 (residues 367-388) was found to have no regular, structure in its native form by NMR spectroscopy, but becomes helical when, bound to Ca2+ loaded S100B(betabeta). The three-dimensional structure of, this complex reveals several favorable hydrophobic and electrostatic, interactions between S100B(betabeta) and the p53 peptide in the binding, pocket, where S100B(betabeta) sterically blocks sites of phosphorylation, and acetylation on p53 that are important for transcription activation. | A Ca2+ dependent conformational change in dimeric S100B(betabeta) is, required for it to bind p53 and inhibit phosphorylation of this tumor, suppressor in its C-terminal negative regulatory domain. A peptide derived, from this region of p53 (residues 367-388) was found to have no regular, structure in its native form by NMR spectroscopy, but becomes helical when, bound to Ca2+ loaded S100B(betabeta). The three-dimensional structure of, this complex reveals several favorable hydrophobic and electrostatic, interactions between S100B(betabeta) and the p53 peptide in the binding, pocket, where S100B(betabeta) sterically blocks sites of phosphorylation, and acetylation on p53 that are important for transcription activation. | ||
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| - | ==Disease== | ||
| - | Known diseases associated with this structure: Adrenal cortical carcinoma OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191170 191170]], Breast cancer OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191170 191170]], Colorectal cancer OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191170 191170]], Hepatocellular carcinoma OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191170 191170]], Histiocytoma OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191170 191170]], Li-Fraumeni syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191170 191170]], Multiple malignancy syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191170 191170]], Nasopharyngeal carcinoma OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191170 191170]], Osteosarcoma OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191170 191170]], Pancreatic cancer OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191170 191170]], Thyroid carcinoma OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191170 191170]] | ||
==About this Structure== | ==About this Structure== | ||
| - | 1DT7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1DT7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DT7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: s100b]] | [[Category: s100b]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:42:03 2008'' |
Revision as of 11:42, 23 January 2008
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SOLUTION STRUCTURE OF THE C-TERMINAL NEGATIVE REGULATORY DOMAIN OF P53 IN A COMPLEX WITH CA2+-BOUND S100B(BB)
Overview
A Ca2+ dependent conformational change in dimeric S100B(betabeta) is, required for it to bind p53 and inhibit phosphorylation of this tumor, suppressor in its C-terminal negative regulatory domain. A peptide derived, from this region of p53 (residues 367-388) was found to have no regular, structure in its native form by NMR spectroscopy, but becomes helical when, bound to Ca2+ loaded S100B(betabeta). The three-dimensional structure of, this complex reveals several favorable hydrophobic and electrostatic, interactions between S100B(betabeta) and the p53 peptide in the binding, pocket, where S100B(betabeta) sterically blocks sites of phosphorylation, and acetylation on p53 that are important for transcription activation.
About this Structure
1DT7 is a Protein complex structure of sequences from Rattus norvegicus with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the negative regulatory domain of p53 bound to S100B(betabeta)., Rustandi RR, Baldisseri DM, Weber DJ, Nat Struct Biol. 2000 Jul;7(7):570-4. PMID:10876243
Page seeded by OCA on Wed Jan 23 13:42:03 2008
