1wqs
From Proteopedia
(New page: 200px<br /><applet load="1wqs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wqs, resolution 2.80Å" /> '''Crystal structure of...) |
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| - | [[Image:1wqs.gif|left|200px]]<br /><applet load="1wqs" size=" | + | [[Image:1wqs.gif|left|200px]]<br /><applet load="1wqs" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1wqs, resolution 2.80Å" /> | caption="1wqs, resolution 2.80Å" /> | ||
'''Crystal structure of Norovirus 3C-like protease'''<br /> | '''Crystal structure of Norovirus 3C-like protease'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1WQS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chiba_virus Chiba virus] with HG, TLA and TAR as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1WQS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chiba_virus Chiba virus] with <scene name='pdbligand=HG:'>HG</scene>, <scene name='pdbligand=TLA:'>TLA</scene> and <scene name='pdbligand=TAR:'>TAR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WQS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: oxyanion hole]] | [[Category: oxyanion hole]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:42:05 2008'' |
Revision as of 11:42, 23 January 2008
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Crystal structure of Norovirus 3C-like protease
Overview
Norovirus 3C-like proteases are crucial to proteolytic processing of, norovirus polyproteins. We determined the crystal structure of the 3C-like, protease from Chiba virus, a norovirus, at 2.8-A resolution. An active, site including Cys139 and His30 is present, as is a hydrogen bond network, that stabilizes the active site conformation. In the oxyanion hole, backbone, a structural difference was observed probably upon substrate, binding. A peptide substrate/enzyme model shows that several interactions, between the two components are critical for substrate binding and that the, S1 and S2 sites appropriately accommodate the substrate P1 and P2, residues, respectively. Knowledge of the structure and a previous, mutagenesis study allow us to correlate proteolysis and structure.
About this Structure
1WQS is a Single protein structure of sequence from Chiba virus with , and as ligands. Full crystallographic information is available from OCA.
Reference
A norovirus protease structure provides insights into active and substrate binding site integrity., Nakamura K, Someya Y, Kumasaka T, Ueno G, Yamamoto M, Sato T, Takeda N, Miyamura T, Tanaka N, J Virol. 2005 Nov;79(21):13685-93. PMID:16227288
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