2ale
From Proteopedia
(New page: 200px<br /><applet load="2ale" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ale, resolution 1.80Å" /> '''Crystal structure of...) |
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| - | [[Image:2ale.jpg|left|200px]]<br /><applet load="2ale" size=" | + | [[Image:2ale.jpg|left|200px]]<br /><applet load="2ale" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2ale, resolution 1.80Å" /> | caption="2ale, resolution 1.80Å" /> | ||
'''Crystal structure of yeast RNA splicing factor Snu13p'''<br /> | '''Crystal structure of yeast RNA splicing factor Snu13p'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2ALE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2ALE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ALE OCA]. |
==Reference== | ==Reference== | ||
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[[Category: yeast]] | [[Category: yeast]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:42:06 2008'' |
Revision as of 11:42, 23 January 2008
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Crystal structure of yeast RNA splicing factor Snu13p
Overview
Snu13p is a Saccharomyces cerevisiae protein essential for pre-messenger, RNA splicing and pre-ribosomal RNA processing. Snu13p binds U4 snRNA of, the spliceosome and box C/D snoRNAs of the pre-ribosomal RNA processing, machinery to induce assembly of each ribonucleoprotein complex. Here, we, present structural and biochemical analysis of Snu13p. The crystal, structure of Snu13p reveals a region of the protein which could be, important for protein interaction during ribonucleoprotein assembly. Using, the structure of Snu13p we have designed the first temperature-sensitive, mutants in Snu13p, L67W and I102A. Wild-type and mutant Snu13p proteins, were assayed for binding to U4 snRNA and U3 snoRNA. Both, temperature-sensitive mutants displayed significantly reduced RNA binding, compared to wild-type protein. As the temperature-sensitive mutations are, not in the known RNA binding region of Snu13p this indicates that these, mutants indirectly influence the RNA binding properties of Snu13p. This, work provides insight into Snu13p function during ribonucleoprotein, assembly.
About this Structure
2ALE is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Full crystallographic information is available from OCA.
Reference
Analysis of pre-mRNA and pre-rRNA processing factor Snu13p structure and mutants., Dobbyn HC, McEwan PA, Krause A, Novak-Frazer L, Bella J, O'Keefe RT, Biochem Biophys Res Commun. 2007 Sep 7;360(4):857-62. Epub 2007 Jul 9. PMID:17631273
Page seeded by OCA on Wed Jan 23 13:42:06 2008
Categories: Saccharomyces cerevisiae | Single protein | Bella, J. | Dobbyn, H.C. | Keefe, R.T.O. | McEwan, P.A. | MG | His-tag | Rna | Splicing | Yeast
