This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2g79
From Proteopedia
(New page: 200px<br /> <applet load="2g79" size="450" color="white" frame="true" align="right" spinBox="true" caption="2g79, resolution 1.690Å" /> '''Crystal Structure ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2g79.gif|left|200px]]<br /> | + | [[Image:2g79.gif|left|200px]]<br /><applet load="2g79" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2g79" size=" | + | |
caption="2g79, resolution 1.690Å" /> | caption="2g79, resolution 1.690Å" /> | ||
'''Crystal Structure of the R132K:Y134F Mutant of Cellular Retinoic Acid Binding Protein Type II in Complex with All-Trans-Retinal at 1.69 Angstroms Resolution'''<br /> | '''Crystal Structure of the R132K:Y134F Mutant of Cellular Retinoic Acid Binding Protein Type II in Complex with All-Trans-Retinal at 1.69 Angstroms Resolution'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2G79 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NA, SO4 and RET as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2G79 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=RET:'>RET</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G79 OCA]. |
==Reference== | ==Reference== | ||
| Line 28: | Line 27: | ||
[[Category: x-ray]] | [[Category: x-ray]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:42:25 2008'' |
Revision as of 11:42, 23 January 2008
|
Crystal Structure of the R132K:Y134F Mutant of Cellular Retinoic Acid Binding Protein Type II in Complex with All-Trans-Retinal at 1.69 Angstroms Resolution
Overview
Rational redesign of the binding pocket of Cellular Retinoic Acid Binding, Protein II (CRABPII) has provided a mutant that can bind retinal as a, protonated Schiff base, mimicking the binding observed in rhodopsin. The, reengineering was accomplished through a series of choreographed, manipulations to ultimately orient the reactive species (the epsilon-amino, group of Lys132 and the carbonyl of retinal) in the proper geometry for, imine formation. The guiding principle was to achieve the appropriate, Burgi-Dunitz trajectory for the reaction to ensue. Through, crystallographic analysis of protein mutants incapable of forming the, requisite Schiff base, a highly ordered water molecule was identified as a, key culprit in orienting retinal in a nonconstructive manner. Removal of, the ordered water, along with placing reinforcing mutations to favor the, desired orientation of retinal, led to a triple mutant CRABPII protein, capable of nanomolar binding of retinal as a protonated Schiff base. The, high-resolution crystal structure of all-trans-retinal bound to the, CRABPII triple mutant (1.2 A resolution) unequivocally illustrates the, imine formed between retinal and the protein.
About this Structure
2G79 is a Single protein structure of sequence from Homo sapiens with , and as ligands. Full crystallographic information is available from OCA.
Reference
Protein design: reengineering cellular retinoic acid binding protein II into a rhodopsin protein mimic., Vasileiou C, Vaezeslami S, Crist RM, Rabago-Smith M, Geiger JH, Borhan B, J Am Chem Soc. 2007 May 16;129(19):6140-8. Epub 2007 Apr 21. PMID:17447762
Page seeded by OCA on Wed Jan 23 13:42:25 2008
Categories: Homo sapiens | Single protein | Geiger, J.H. | Vaezeslami, S. | NA | RET | SO4 | Beta barrel | Crabpii | Crystallography | High resolution | Retinal | Retinoic acid | Retinoids | X-ray
