2qox
From Proteopedia
(New page: 200px<br /><applet load="2qox" size="450" color="white" frame="true" align="right" spinBox="true" caption="2qox, resolution 3.93Å" /> '''Crystal structure of...) |
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| - | [[Image:2qox.gif|left|200px]]<br /><applet load="2qox" size=" | + | [[Image:2qox.gif|left|200px]]<br /><applet load="2qox" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2qox, resolution 3.93Å" /> | caption="2qox, resolution 3.93Å" /> | ||
'''Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 50S subunit of the second 70S ribosome. The entire crystal structure contains two 70S ribosomes.'''<br /> | '''Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 50S subunit of the second 70S ribosome. The entire crystal structure contains two 70S ribosomes.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2QOX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteria Bacteria] with MG and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2QOX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteria Bacteria] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rna-protein complex]] | [[Category: rna-protein complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:44:06 2008'' |
Revision as of 11:44, 23 January 2008
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Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 50S subunit of the second 70S ribosome. The entire crystal structure contains two 70S ribosomes.
Overview
The widely used antibiotic spectinomycin inhibits bacterial protein, synthesis by blocking translocation of messenger RNA and transfer RNAs on, the ribosome. Here, we show that in crystals of the Escherichia coli 70S, ribosome spectinomycin binding traps a distinct swiveling state of the, head domain of the small ribosomal subunit. Spectinomycin also alters the, rate and completeness of reverse translocation in vitro. These structural, and biochemical data indicate that in solution spectinomycin sterically, blocks swiveling of the head domain of the small ribosomal subunit and, thereby disrupts the translocation cycle.
About this Structure
2QOX is a Protein complex structure of sequences from Bacteria with and as ligands. Full crystallographic information is available from OCA.
Reference
A steric block in translation caused by the antibiotic spectinomycin., Borovinskaya MA, Shoji S, Holton JM, Fredrick K, Cate JH, ACS Chem Biol. 2007 Aug 17;2(8):545-52. Epub 2007 Aug 10. PMID:17696316
Page seeded by OCA on Wed Jan 23 13:44:06 2008
Categories: Bacteria | Protein complex | Borovinskaya, M.A. | Cate, J.H.D. | Fredrick, K. | Holton, J.M. | Shoji, S. | MG | ZN | Ribosome | Rna-protein complex
