2nm3
From Proteopedia
(New page: 200px<br /><applet load="2nm3" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nm3, resolution 1.68Å" /> '''Crystal structure of...) |
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caption="2nm3, resolution 1.68Å" /> | caption="2nm3, resolution 1.68Å" /> | ||
'''Crystal structure of dihydroneopterin aldolase from S. aureus in complex with (1S,2S)-monapterin at 1.68 angstrom resolution'''<br /> | '''Crystal structure of dihydroneopterin aldolase from S. aureus in complex with (1S,2S)-monapterin at 1.68 angstrom resolution'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NM3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with ACT and MPU as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Dihydroneopterin_aldolase Dihydroneopterin aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.25 4.1.2.25] Full crystallographic information is available from [http:// | + | 2NM3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=MPU:'>MPU</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Dihydroneopterin_aldolase Dihydroneopterin aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.25 4.1.2.25] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NM3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:46:07 2008'' |
Revision as of 11:46, 23 January 2008
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Crystal structure of dihydroneopterin aldolase from S. aureus in complex with (1S,2S)-monapterin at 1.68 angstrom resolution
Overview
Dihydroneopterin aldolase (DHNA) catalyzes the conversion of, 7,8-dihydroneopterin (DHNP) to 6-hydroxymethyl-7,8-dihydropterin (HP) and, the epimerization of DHNP to 7,8-dihydromonopterin (DHMP). Although, crystal structures of the enzyme from several microorganisms have been, reported, no structural information is available about the critical, interactions between DHNA and the trihydroxypropyl moiety of the, substrate, which undergoes bond cleavage and formation. Here, we present, the structures of Staphylococcus aureus DHNA (SaDHNA) in complex with, neopterin (NP, an analog of DHNP) and with monapterin (MP, an analog of, DHMP), filling the gap in the structural analysis of the enzyme. In, combination with previously reported SaDHNA structures in its ligand-free, form (PDB entry 1DHN) and in complex with HP (PDB entry 2DHN), four, snapshots for the catalytic center assembly along the reaction pathway can, be derived, advancing our knowledge about the molecular mechanism of, SaDHNA-catalyzed reactions. An additional step appears to be necessary for, the epimerization of DHMP to DHNP. Three active site residues (E22, K100, and Y54) function coordinately during catalysis: together, they organize, the catalytic center assembly, and individually, each plays a central role, at different stages of the catalytic cycle.
About this Structure
2NM3 is a Single protein structure of sequence from Staphylococcus aureus with and as ligands. Active as Dihydroneopterin aldolase, with EC number 4.1.2.25 Full crystallographic information is available from OCA.
Reference
Structural basis for the aldolase and epimerase activities of Staphylococcus aureus dihydroneopterin aldolase., Blaszczyk J, Li Y, Gan J, Yan H, Ji X, J Mol Biol. 2007 Apr 20;368(1):161-9. Epub 2007 Feb 9. PMID:17331536
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Categories: Dihydroneopterin aldolase | Single protein | Staphylococcus aureus | Blaszczyk, J. | Ji, X. | Yan, H. | ACT | MPU | 7 | 8-dihydromonapterin | Dhna | Drug design | Lyase | Monapterin | Neopterin | Substrate complex | X-ray crystallography
